Analysis of acrylamide in coffee and dietary exposure to acrylamide from coffee

Kit Granby, Sisse Fagt

Research output: Contribution to journalConference articleResearchpeer-review


An analytical method for analysing acrylamide in coffee was validated. The analysis of prepared coffee includes a comprehensive clean-up using multimode solid-phase extraction (SPE) by automatic SPE equipment and detection by liquid chromatography tandem mass spectrometry using electrospray in the positive mode. The recoveries of acrylamide in ready-to-drink coffee spiked with 5 and 10 mug l(-1) were 96 +/- 14% and 100 +/- 8%, respectively. Within laboratory reproducibility for the same spiking levels were 14% and 9%, respectively. Coffee samples (n = 25) prepared twice by coffee machines and twice by a French Press Cafetiere coffee maker contained 8 +/- 3 mug l(-1) and 9 +/- 3 mug l(-1) acrylamide. Five ready-to-drink instant coffee prepared twice contained 8 +/- 2 mug l(-1). Hence, the results do not show significant differences in the acrylamide contents in ready-to-drink coffee prepared by coffee machine, French Press or from instant coffee. Medium roasted coffee contained more acrylamide (similar to10 mug l(-1)) than dark roasted coffee (similar to5 mug l(-1)). Males aged 35-45 years, drinking on average 1.11 coffee per day are exposed to the highest doses of acrylamide from coffee. The dietary intake of acrylamide from coffee comprises, on an average, 10 mug day(-1) for males and 9 mug day(-1) for females aged 35-45 years. Probabilistic modelling of the exposure of Danish consumers (all adults) to acrylamide from coffee shows a mean exposure of 6.5 mug day(-1) and a 95 percentile of 18 mug day(-1). (C) 2004 Elsevier B.V. All rights reserved.
Original languageEnglish
JournalAnalytica Chimica Acta
Issue number1-2
Pages (from-to)177-182
Publication statusPublished - 2004
Event1st International Symposium on Recent Advances in Food Analysis - Prague, Czech Republic
Duration: 5 Nov 20037 Nov 2003


Conference1st International Symposium on Recent Advances in Food Analysis
Country/TerritoryCzech Republic


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