Abstract
The aim of this study was to analyse the genetic diversity among Clostridium perfringens isolates from Danish broiler chickens since both sick and presumably healthy animals were investigated. Isolates (n = 279) collected from chickens from 25 farms were analysed by pulsed-field gel electrophoresis (PFGE) with the restriction enzyme SmaI. A high genetic diversity was found. Isolates with different PFGE types were toxin typed by PCR and all were found to be of type A. The results showed that healthy broiler chickens carried several different C. perfringens clones both within a flock and even within individual birds, whereas flocks suffering from necrotic enteritis (NE) or cholangio-hepatitis carried only one or two clones.
| Original language | English |
|---|---|
| Journal | Veterinary Microbiology |
| Volume | 94 |
| Issue number | 3 |
| Pages (from-to) | 257-266 |
| ISSN | 0378-1135 |
| DOIs | |
| Publication status | Published - 2003 |
| Externally published | Yes |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- chicken
- pulsed-field gel electrophoresis
- Clostridium perfringens
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