Analysis by pulsed-field gel electrophoresis of the genetic diversity among Clostridium perfringens isolates from chickens

B. Nauerby; Karl Pedersen; Mogens Madsen

doi:10.1016/S0378-1135(03)00118-4

Analysis by pulsed-field gel electrophoresis of the genetic diversity among Clostridium perfringens isolates from chickens

B. Nauerby, Karl Pedersen, Mogens Madsen

Danish Veterinary Institute

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The aim of this study was to analyse the genetic diversity among Clostridium perfringens isolates from Danish broiler chickens since both sick and presumably healthy animals were investigated. Isolates (n = 279) collected from chickens from 25 farms were analysed by pulsed-field gel electrophoresis (PFGE) with the restriction enzyme SmaI. A high genetic diversity was found. Isolates with different PFGE types were toxin typed by PCR and all were found to be of type A. The results showed that healthy broiler chickens carried several different C. perfringens clones both within a flock and even within individual birds, whereas flocks suffering from necrotic enteritis (NE) or cholangio-hepatitis carried only one or two clones.

Original language	English
Journal	Veterinary Microbiology
Volume	94
Issue number	3
Pages (from-to)	257-266
ISSN	0378-1135
DOIs	https://doi.org/10.1016/S0378-1135(03)00118-4
Publication status	Published - 2003
Externally published	Yes

Keywords

chicken
pulsed-field gel electrophoresis
Clostridium perfringens

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0378-1135(03)00118-4

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title = "Analysis by pulsed-field gel electrophoresis of the genetic diversity among Clostridium perfringens isolates from chickens",

abstract = "The aim of this study was to analyse the genetic diversity among Clostridium perfringens isolates from Danish broiler chickens since both sick and presumably healthy animals were investigated. Isolates (n = 279) collected from chickens from 25 farms were analysed by pulsed-field gel electrophoresis (PFGE) with the restriction enzyme SmaI. A high genetic diversity was found. Isolates with different PFGE types were toxin typed by PCR and all were found to be of type A. The results showed that healthy broiler chickens carried several different C. perfringens clones both within a flock and even within individual birds, whereas flocks suffering from necrotic enteritis (NE) or cholangio-hepatitis carried only one or two clones.",

keywords = "chicken, pulsed-field gel electrophoresis, Clostridium perfringens",

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doi = "10.1016/S0378-1135(03)00118-4",

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T1 - Analysis by pulsed-field gel electrophoresis of the genetic diversity among Clostridium perfringens isolates from chickens

AU - Nauerby, B.

AU - Pedersen, Karl

AU - Madsen, Mogens

PY - 2003

Y1 - 2003

N2 - The aim of this study was to analyse the genetic diversity among Clostridium perfringens isolates from Danish broiler chickens since both sick and presumably healthy animals were investigated. Isolates (n = 279) collected from chickens from 25 farms were analysed by pulsed-field gel electrophoresis (PFGE) with the restriction enzyme SmaI. A high genetic diversity was found. Isolates with different PFGE types were toxin typed by PCR and all were found to be of type A. The results showed that healthy broiler chickens carried several different C. perfringens clones both within a flock and even within individual birds, whereas flocks suffering from necrotic enteritis (NE) or cholangio-hepatitis carried only one or two clones.

AB - The aim of this study was to analyse the genetic diversity among Clostridium perfringens isolates from Danish broiler chickens since both sick and presumably healthy animals were investigated. Isolates (n = 279) collected from chickens from 25 farms were analysed by pulsed-field gel electrophoresis (PFGE) with the restriction enzyme SmaI. A high genetic diversity was found. Isolates with different PFGE types were toxin typed by PCR and all were found to be of type A. The results showed that healthy broiler chickens carried several different C. perfringens clones both within a flock and even within individual birds, whereas flocks suffering from necrotic enteritis (NE) or cholangio-hepatitis carried only one or two clones.

KW - chicken

KW - pulsed-field gel electrophoresis

KW - Clostridium perfringens

U2 - 10.1016/S0378-1135(03)00118-4

DO - 10.1016/S0378-1135(03)00118-4

M3 - Journal article

SN - 0378-1135

VL - 94

SP - 257

EP - 266

JO - Veterinary Microbiology

JF - Veterinary Microbiology

IS - 3

ER -

Analysis by pulsed-field gel electrophoresis of the genetic diversity among Clostridium perfringens isolates from chickens

Abstract

Keywords

UN SDGs

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