Analysis by pulsed-field gel electrophoresis of the genetic diversity among Clostridium perfringens isolates from chickens

B. Nauerby, Karl Pedersen, Mogens Madsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The aim of this study was to analyse the genetic diversity among Clostridium perfringens isolates from Danish broiler chickens since both sick and presumably healthy animals were investigated. Isolates (n = 279) collected from chickens from 25 farms were analysed by pulsed-field gel electrophoresis (PFGE) with the restriction enzyme SmaI. A high genetic diversity was found. Isolates with different PFGE types were toxin typed by PCR and all were found to be of type A. The results showed that healthy broiler chickens carried several different C. perfringens clones both within a flock and even within individual birds, whereas flocks suffering from necrotic enteritis (NE) or cholangio-hepatitis carried only one or two clones.
Original languageEnglish
JournalVeterinary Microbiology
Volume94
Issue number3
Pages (from-to)257-266
ISSN0378-1135
DOIs
Publication statusPublished - 2003
Externally publishedYes

Keywords

  • chicken
  • pulsed-field gel electrophoresis
  • Clostridium perfringens

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