Analyses of  restriction fragment patterns (RFPs) and pathogenicity in baby mice of equine herpesvirus 1 and 4 (EHV-1 and EHV-4) strains circulating in Danish horses

V. Palfi; Laurids Siig Christensen

doi:10.1016/0378-1135(95)00053-D

Analyses of restriction fragment patterns (RFPs) and pathogenicity in baby mice of equine herpesvirus 1 and 4 (EHV-1 and EHV-4) strains circulating in Danish horses

V. Palfi, Laurids Siig Christensen

University of Copenhagen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Twentyfive strains of equine herpesvirus 1 (EHV-1) and one strain of equine herpesvirus 4 (ERV-4) isolated from material from various clinical cases in Denmark, together with reference EHV-1 and EHV-4 strains, were compared by restriction fragment pattern (RFP) analysis and inoculation of baby mice. The RFP analyses revealed that all EHV-1 strains belonged to genome type Ip. Four fetal isolates exhibited genomic characteristics that have been suggested as specific markers of the attenuated strain Rac H, widely used as a live vaccine. As the use of live vaccines against EHV-1 and EHV-4 has never been allowed in Denmark, it is assumed that Rac H derivatives have been acquired from visiting horses and thus are now circulating in the horse population. Baby mice inoculation revealed that four biotypes could be distinguished on the basis of pathogenicity. However, no strict correlation with pathogenicity in the natural host was seen.

Original language	English
Journal	Veterinary Microbiology
Volume	47
Issue number	1-2
Pages (from-to)	199-204
ISSN	0378-1135
DOIs	https://doi.org/10.1016/0378-1135(95)00053-D
Publication status	Published - 1995
Externally published	Yes

Keywords

Equine herpesvirus
Horse
Restriction fragment pattern analysis
Vaccine

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10.1016/0378-1135(95)00053-D

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title = "Analyses of restriction fragment patterns (RFPs) and pathogenicity in baby mice of equine herpesvirus 1 and 4 (EHV-1 and EHV-4) strains circulating in Danish horses",

abstract = "Twentyfive strains of equine herpesvirus 1 (EHV-1) and one strain of equine herpesvirus 4 (ERV-4) isolated from material from various clinical cases in Denmark, together with reference EHV-1 and EHV-4 strains, were compared by restriction fragment pattern (RFP) analysis and inoculation of baby mice. The RFP analyses revealed that all EHV-1 strains belonged to genome type Ip. Four fetal isolates exhibited genomic characteristics that have been suggested as specific markers of the attenuated strain Rac H, widely used as a live vaccine. As the use of live vaccines against EHV-1 and EHV-4 has never been allowed in Denmark, it is assumed that Rac H derivatives have been acquired from visiting horses and thus are now circulating in the horse population. Baby mice inoculation revealed that four biotypes could be distinguished on the basis of pathogenicity. However, no strict correlation with pathogenicity in the natural host was seen.",

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AU - Christensen, Laurids Siig

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AB - Twentyfive strains of equine herpesvirus 1 (EHV-1) and one strain of equine herpesvirus 4 (ERV-4) isolated from material from various clinical cases in Denmark, together with reference EHV-1 and EHV-4 strains, were compared by restriction fragment pattern (RFP) analysis and inoculation of baby mice. The RFP analyses revealed that all EHV-1 strains belonged to genome type Ip. Four fetal isolates exhibited genomic characteristics that have been suggested as specific markers of the attenuated strain Rac H, widely used as a live vaccine. As the use of live vaccines against EHV-1 and EHV-4 has never been allowed in Denmark, it is assumed that Rac H derivatives have been acquired from visiting horses and thus are now circulating in the horse population. Baby mice inoculation revealed that four biotypes could be distinguished on the basis of pathogenicity. However, no strict correlation with pathogenicity in the natural host was seen.

KW - Equine herpesvirus

KW - Horse

KW - Restriction fragment pattern analysis

KW - Vaccine

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