An Overview of Cell-Based Assay Platforms for the Solute Carrier Family of Transporters

Vojtech Dvorak, Tabea Wiedmer, Alvaro Ingles-Prieto, Patrick Altermatt, Helena Batoulis, Felix Bärenz, Eckhard Bender, Daniela Digles, Franz Dürrenberger, Laura H. Heitman, Adriaan P. IJzerman, Douglas B. Kell, Stefanie Kickinger, Daniel Körzö, Philipp Leippe, Thomas Licher, Vania Manolova, Riccardo Rizzetto, Francesca Sassone, Lia ScarabottoloAvner Schlessinger, Vanessa Schneider, Hubert J. Sijben, Anna Lena Steck, Hanna Sundström, Sara Tremolada, Maria Wilhelm, Marina Wright Muelas, Diana Zindel, Claire M. Steppan, Giulio Superti-Furga*

*Corresponding author for this work

Research output: Contribution to journalReviewResearchpeer-review

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Abstract

The solute carrier (SLC) superfamily represents the biggest family of transporters with important roles in health and disease. Despite being attractive and druggable targets, the majority of SLCs remains understudied. One major hurdle in research on SLCs is the lack of tools, such as cell-based assays to investigate their biological role and for drug discovery. Another challenge is the disperse and anecdotal information on assay strategies that are suitable for SLCs. This review provides a comprehensive overview of state-of-the-art cellular assay technologies for SLC research and discusses relevant SLC characteristics enabling the choice of an optimal assay technology. The Innovative Medicines Initiative consortium RESOLUTE intends to accelerate research on SLCs by providing the scientific community with high-quality reagents, assay technologies and data sets, and to ultimately unlock SLCs for drug discovery.

Original languageEnglish
Article number722889
JournalFrontiers in Pharmacology
Volume12
Number of pages31
ISSN1663-9812
DOIs
Publication statusPublished - 10 Aug 2021

Bibliographical note

Funding Information:
Assay technologies presented here are applied and developed further in the RESOLUTE consortium, a public-private partnership funded by the Innovative Medicines Initiative (IMI) of the European Union. RESOLUTE aims at empowering the research community with open-access reagents and data to unlock the SLC family for drug discovery. One of the main goals of RESOLUTE is to systematically assess the suitability of transport assay technologies for individual SLCs and develop them further (Superti-Furga et al., 2020). We are expecting to update this review with experience gained throughout the project.

Funding Information:
This work is part of the RESOLUTE project that has received funding from the Innovative Medicines Initiative 2 Joint Undertaking under Grant Agreement No. 777372. This Joint Undertaking receives support from the European Union’s

Publisher Copyright:
© Copyright © 2021 Dvorak, Wiedmer, Ingles-Prieto, Altermatt, Batoulis, Bärenz, Bender, Digles, Dürrenberger, Heitman, IJzerman, Kell, Kickinger, Körzö, Leippe, Licher, Manolova, Rizzetto, Sassone, Scarabottolo, Schlessinger, Schneider, Sijben, Steck, Sundström, Tremolada, Wilhelm, Wright Muelas, Zindel, Steppan and Superti-Furga.

Keywords

  • Cell-based assay
  • Chemical screening
  • Drug discovery
  • SLC
  • Solute carrier
  • Transporters

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