An optimized method for fatty acid analysis, including quantification of trans fatty acids, in human adipose tissue by gas-liquid chromatography

Anette Bysted, S Cold, Gunhild Kofoed Hølmer

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    Considering the need for a quick direct method for measurement of the fatty acid composition including trans isomers ofhuman adipose tissue we have developed a procedure using gas-liquid chromatography (GLC) alone, which is thussuitable for validation of fatty acid status in epidemiological studies. Fatty acids ranging in carbon number from 12 to 22and with 0-6 double bonds were resolved and identified by capillary column GLC with a temperature program starting at150 degrees C. Following injection, the oven temperature was increased at a rate of 3 degrees C/min to 200 degrees C,then held constant for 25 min, and finally raised at 25 degrees C/min to 225 degrees C. The trans and cis isomers of18:1 were well separated from each other, as shown by silver-ion thin-layer chromatography. Verification by standardsshowed that the trans 18:1 isomers with a double bond in position 12 or lower were separated from the cis 18:1 isomerswith a double bond in position 6 or higher. As the adipose tissue samples contained only small amounts of the 13t-, 14t-and 15t-18:1 isomers and the 4c- and 5c-18:1 isomers the overlapping was found to be minimal. The GLC method mayalso be valuable for determining the fatty acid profiles including total trans in other tissues.
    Original languageEnglish
    JournalScandinavian Journal of Clinical Laboratory Investigation
    Pages (from-to)205-214
    Publication statusPublished - 1999

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