An integrated membrane system for the biocatalytic production of 3′-sialyllactose from dairy by-products

Jianquan Luo, Rune Thorbjørn Nordvang, Sofie Thage Morthensen, Birgitte Zeuner, Anne S. Meyer, Jørn Dalgaard Mikkelsen, Manuel Pinelo

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

An integrated membrane system was investigated for the production of 30-sialyllactose by an engineered sialidase using casein glycomacropeptide (CGMP) and lactose as substrates. CGMP was purified by ultrafiltration (UF) to remove any small molecules present and then an enzymatic membrane reactor (EMR) was used to separate the product and reuse the enzyme. A PLCC regenerated cellulose membrane was found to be the most suitable for both the UF purification and EMR. Subsequently, nanofiltration (NF) was conducted to increase the purity of the 30-sialyllactose by removing the excess lactose present. The NTR7450 membrane outperformed others in NF due to its high retention of 30-sialyllactose (98%) and relatively low rejection of lactose (40%). The lactose in the permeate could be concentrated by the NF45 membrane and recycled into the EMR. The described integrated membrane system enables a more economic and efficient enzymatic production of 30-sialyllactose.
Original languageEnglish
JournalBioresource Technology
Volume166
Pages (from-to)9-16
ISSN0960-8524
DOIs
Publication statusPublished - 2014

Keywords

  • Ultrafiltration
  • Nanofiltration
  • Enzyme
  • EMR
  • Human milk oligosaccharides

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