An Industrial Perspective on Scale-Down Challenges Using Miniaturized Bioreactors

Tannaz Tajsoleiman, Lisa Mears, Ulrich Krühne, Krist V. Gernaey, Sjef Cornelissen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Miniaturized stirred bioreactors (MSBRs) are gaining popularity as a cost-effective approach to scale-down experimentation. However, realizing conditions that reflect the large-scale process accurately can be challenging. This article highlights common challenges of using MSBRs for scale-down. The fundamental difference between oxygen mass transfer coefficient (kLa) and oxygen transfer rate scaling is addressed and the difficulty of achieving turbulent flow and industrially relevant tip speeds is described. More practical challenges of using MSBR systems for scale-down are also discussed, including the risk of vortex formation, changed volume dynamics, and wall growth. By highlighting these challenges, the article aims to create more awareness of these difficulties and to contribute to improved design of scale-down experiments.
Original languageEnglish
JournalTrends in Biotechnology
Volume37
Issue number7
Pages (from-to)697-706
Number of pages10
ISSN0167-7799
DOIs
Publication statusPublished - 2019

Keywords

  • Scale-down
  • Scale-up
  • Miniaturized stirred bioreactors
  • High throughput
  • Fermentation
  • Process development

Cite this

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title = "An Industrial Perspective on Scale-Down Challenges Using Miniaturized Bioreactors",
abstract = "Miniaturized stirred bioreactors (MSBRs) are gaining popularity as a cost-effective approach to scale-down experimentation. However, realizing conditions that reflect the large-scale process accurately can be challenging. This article highlights common challenges of using MSBRs for scale-down. The fundamental difference between oxygen mass transfer coefficient (kLa) and oxygen transfer rate scaling is addressed and the difficulty of achieving turbulent flow and industrially relevant tip speeds is described. More practical challenges of using MSBR systems for scale-down are also discussed, including the risk of vortex formation, changed volume dynamics, and wall growth. By highlighting these challenges, the article aims to create more awareness of these difficulties and to contribute to improved design of scale-down experiments.",
keywords = "Scale-down, Scale-up, Miniaturized stirred bioreactors, High throughput, Fermentation, Process development",
author = "Tannaz Tajsoleiman and Lisa Mears and Ulrich Kr{\"u}hne and Gernaey, {Krist V.} and Sjef Cornelissen",
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An Industrial Perspective on Scale-Down Challenges Using Miniaturized Bioreactors. / Tajsoleiman, Tannaz; Mears, Lisa; Krühne, Ulrich; Gernaey, Krist V.; Cornelissen, Sjef.

In: Trends in Biotechnology, Vol. 37, No. 7, 2019, p. 697-706.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - An Industrial Perspective on Scale-Down Challenges Using Miniaturized Bioreactors

AU - Tajsoleiman, Tannaz

AU - Mears, Lisa

AU - Krühne, Ulrich

AU - Gernaey, Krist V.

AU - Cornelissen, Sjef

PY - 2019

Y1 - 2019

N2 - Miniaturized stirred bioreactors (MSBRs) are gaining popularity as a cost-effective approach to scale-down experimentation. However, realizing conditions that reflect the large-scale process accurately can be challenging. This article highlights common challenges of using MSBRs for scale-down. The fundamental difference between oxygen mass transfer coefficient (kLa) and oxygen transfer rate scaling is addressed and the difficulty of achieving turbulent flow and industrially relevant tip speeds is described. More practical challenges of using MSBR systems for scale-down are also discussed, including the risk of vortex formation, changed volume dynamics, and wall growth. By highlighting these challenges, the article aims to create more awareness of these difficulties and to contribute to improved design of scale-down experiments.

AB - Miniaturized stirred bioreactors (MSBRs) are gaining popularity as a cost-effective approach to scale-down experimentation. However, realizing conditions that reflect the large-scale process accurately can be challenging. This article highlights common challenges of using MSBRs for scale-down. The fundamental difference between oxygen mass transfer coefficient (kLa) and oxygen transfer rate scaling is addressed and the difficulty of achieving turbulent flow and industrially relevant tip speeds is described. More practical challenges of using MSBR systems for scale-down are also discussed, including the risk of vortex formation, changed volume dynamics, and wall growth. By highlighting these challenges, the article aims to create more awareness of these difficulties and to contribute to improved design of scale-down experiments.

KW - Scale-down

KW - Scale-up

KW - Miniaturized stirred bioreactors

KW - High throughput

KW - Fermentation

KW - Process development

U2 - 10.1016/j.tibtech.2019.01.002

DO - 10.1016/j.tibtech.2019.01.002

M3 - Journal article

VL - 37

SP - 697

EP - 706

JO - Trends in Biotechnology

JF - Trends in Biotechnology

SN - 0167-7799

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ER -