An experimental infection model for reproduction of calf pneumonia with bovine respiratory syncytial virus (BRSV) based on one combined exposure of calves

Kirsten Tjørnehøj, Åse Uttenthal, B. Viuff, Lars Erik Larsen, C. Rontved, L. Ronsholt

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Bovine respiratory syncytial virus (BRSV) has been recognised as an important pathogen in calf pneumonia for 30 years, but surprisingly few effective infection models for studies of the immune response and the pathogenesis in the natural host have been established. We present a reproducible experimental infection model for BRSV in 2-5-month-old, conventionally reared Jersey calves. Thirty-four colostrum-fed calves were inoculated once by aerosol and intratracheal injection with BRSV. Respiratory disease was recorded in 91% of the BRSV-inoculated calves, 72% had an accompanying rise in rectal temperature and 83% exhibited >5%, consolidation of the lung tissue. The disease closely resembled natural outbreaks of BRSV-related pneumonia, and detection of BRSV in nasal secretions and lung tissues confirmed the primary role of BRSV. Nine mock-inoculated control calves failed to develop respiratory disease. This model is a valuable tool for the study of the pathogenesis of BRSV and for vaccine efficacy studies.
    Original languageEnglish
    JournalResearch in Veterinary Science
    Volume74
    Issue number1
    Pages (from-to)55-65
    ISSN0034-5288
    DOIs
    Publication statusPublished - 2003

    Keywords

    • respiratory syncytial virus
    • bovine
    • infection model
    • BRSV
    • pneumonia
    • calves

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