An Expanded Heterologous GAL Promoter Collection for Diauxie-Inducible Expression in Saccharomyces cerevisiae

Bingyin Peng*, Rebecca J. Wood, Lars K. Nielsen, Claudia E. Vickers

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The GAL promoters are applied in metabolic engineering and synthetic biology to control gene expression in the budding yeast Saccharomyces cerevisiae. In ga1800 background strains, they show diauxie-inducible expression, a feature beneficial in metabolic pathway optimization. However, only a limited number of GAL promoters have been characterized and are available for engineering purposes. Multiple uses of the same promoters can result in genetic instability in engineered strains due to homologous recombination. Here, 11 GALI/2 promoters from other Saccharomyces species were isolated and characterized in S. cerevisiae. They exhibited diauxie-inducible expression patterns with low strength in exponential growth phase and induction in the ethanol growth phase. These promoters represent an expansion to the collection of GAL promoters available for genetic engineering in S. cerevisiae, including an increased diversity of expression levels. This provides the capacity for increased numbers of genetic manipulations with a lower risk of genetic instability.
Original languageEnglish
JournalA C S Synthetic Biology
Volume7
Issue number2
Pages (from-to)748-751
ISSN2161-5063
DOIs
Publication statusPublished - 2018

Keywords

  • Galactose-inducible promoter
  • Saccharomyces species
  • Autoinducible expression
  • Promoter library
  • Metabolic engineering
  • Synthetic biology

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