An epifluorescence microscopy method for generalized polarization imaging

Jesper Søndergaard Hansen, Claus Helix Nielsen

Research output: Contribution to journalJournal articleResearchpeer-review


Generalized polarization (GP) microscopy represents an excellent tool to study lipid–lipid and lipid–protein interactions in situ and in vitro. Here, we present an efficient and cost effective method to perform GP microscopy using a standard light-emitting diode (LED) epifluorescence microscope equipped with a digital color camera.
Original languageEnglish
JournalBiochemical and Biophysical Research Communications
Issue number4
Pages (from-to)686-690
Publication statusPublished - 2011


  • Generalized polarization
  • Giant unilamellar vesicles
  • Microscopy
  • Epifluorescence

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