TY - JOUR
T1 - An engineered monomer binding-protein for α-synuclein efficiently inhibits the proliferation of amyloid fibrils
AU - Agerschou, Emil Dandanell
AU - Flagmeier, Patrick
AU - Saridaki, Theodora
AU - Galvagnion, Céline
AU - Komnig, Daniel
AU - Heid, Laetitia
AU - Prasad, Vibha
AU - Shaykhalishahi, Hamed
AU - Willbold, Dieter
AU - Dobson, Christopher M.
AU - Voigt, Aaron
AU - Falkenburger, Bjoern
AU - Hoyer, Wolfgang
AU - Buell, Alexander K.
PY - 2019
Y1 - 2019
N2 - Removing or preventing the formation of α-synuclein aggregates is a plausible strategy against Parkinson’s disease. To this end, we have engineered the β-wrap in AS69 to bind monomeric α-synuclein with high affinity. In cultured cells, AS69 reduced the self-interaction of a synuclein and formation of visible α-synuclein aggregates. In flies, AS69 reduced α-synuclein aggregates and the locomotor deficit resulting from α-synuclein expression in neuronal cells. In biophysical experiments in vitro, AS69 highly sub-stoichiometrically inhibited both primary and autocatalytic secondary nucleation processes, even in the presence of a large excess of monomer. We present evidence that the AS69-α-synuclein complex, rather than the free AS69, is the inhibitory species responsible for sub-stoichiometric inhibition of secondary nucleation. These results represent a new paradigm that high affinity monomer binders can lead to strongly sub-stoichiometric inhibition of nucleation processes.
AB - Removing or preventing the formation of α-synuclein aggregates is a plausible strategy against Parkinson’s disease. To this end, we have engineered the β-wrap in AS69 to bind monomeric α-synuclein with high affinity. In cultured cells, AS69 reduced the self-interaction of a synuclein and formation of visible α-synuclein aggregates. In flies, AS69 reduced α-synuclein aggregates and the locomotor deficit resulting from α-synuclein expression in neuronal cells. In biophysical experiments in vitro, AS69 highly sub-stoichiometrically inhibited both primary and autocatalytic secondary nucleation processes, even in the presence of a large excess of monomer. We present evidence that the AS69-α-synuclein complex, rather than the free AS69, is the inhibitory species responsible for sub-stoichiometric inhibition of secondary nucleation. These results represent a new paradigm that high affinity monomer binders can lead to strongly sub-stoichiometric inhibition of nucleation processes.
U2 - 10.7554/eLife.46112
DO - 10.7554/eLife.46112
M3 - Journal article
C2 - 31389332
AN - SCOPUS:85071785283
VL - 8
JO - eLife
JF - eLife
SN - 2050-084X
M1 - e46112
ER -