Abstract
We present the successful integration of an electroosmotic flow pump and a patch-clamp orifice in the same package. When experiments are performed the electroosmotic pump is used both to position e.g. a single HEK293 cell on a micron-sized orifice and later to rupture the cell membrane, to bring the cell in the whole-cell configuration. In this configuration we are able to perform high quality electrophysiological ion-channel measurements. In the experiments we have used a sieve geometry for the pump. This design is a particularly promising candidate for such an integration scheme. The sieve pumps have relatively low volumetric flow rates, but are able to supply relatively high stall pressures (>200mbar), which make the sieve pumps well suited to work on a load with a high flow resistance e.g. a patch-clamp orifice. In the following we present a characterisation of such electroosmotic sieve pumps. For our system we were able to determine the zeta-potential of the glass/electrolyte interface of to be well above 10mV. Furthermore we also present ion-channel measurements performed on this integrated platform.
Original language | English |
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Title of host publication | BOSTON TRANSDUCERS'03 : DIGEST OF TECHNICAL PAPERS |
Volume | Volume 1 & 2 |
Publisher | IEEE |
Publication date | 2003 |
Pages | 1059-1062 |
ISBN (Print) | 0-7803-7731-1 |
Publication status | Published - 2003 |
Externally published | Yes |
Event | 12th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers) - Boston, United States Duration: 9 Jun 2003 → 12 Jun 2003 |
Conference
Conference | 12th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers) |
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Country/Territory | United States |
City | Boston |
Period | 09/06/2003 → 12/06/2003 |