An effect-directed strategy for characterizing emerging chemicals in food contact materials made from paper and board

Anna Kjerstine Rosenmai, Linda Bengtström, Camilla Taxvig, Xenia Trier, Jens Højslev Petersen, Terje Svingen, Mona-Lise Binderup, Barbare Medea Akuce van Vugt-Lussenburg, Marianne Dybdahl, Kit Granby, Anne Marie Vinggaard

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Abstract

Food contact materials (FCM) are any type of item intended to come into contact with foods and thus represent a potential source for human exposure to chemicals. Regarding FCMs made of paper and board, information pertaining to their chemical constituents and the potential impacts on human health remains scarce, which hampers safety evaluation. We describe an effect-directed strategy to identify and characterize emerging chemicals in paper and board FCMs. Twenty FCMs were tested in eight reporter gene assays, including assays for the AR, ER, AhR, PPARγ, Nrf2 and p53, as well as mutagenicity. All FCMs exhibited activities in at least one assay. As proof-of-principle, FCM samples obtained from a sandwich wrapper and a pizza box were carried through a complete step-by-step multi-tiered approach. The pizza box exhibited ER activity, likely caused by the presence of bisphenol A, dibutyl phthalate, and benzylbutyl phthalate. The sandwich wrapper exhibited AR antagonism, likely caused by abietic acid and dehydroabietic acid. Migration studies confirmed that the active chemicals can transfer from FCMs to food simulants. In conclusion, we report an effect-directed strategy that can identify hazards posed by FCMs made from paper and board, including the identification of the chemical(s) responsible for the observed activity.
Original languageEnglish
JournalFood and Chemical Toxicology
Volume106
Issue numberPart A
Pages (from-to)250-259
ISSN0278-6915
DOIs
Publication statusPublished - 2017

Keywords

  • Abietic acid
  • Bisphenol A
  • Effect-directed analysis
  • Food packaging materials
  • Hazard identification
  • In vitro tests
  • Paper and board
  • Phthalates

Cite this

Rosenmai, Anna Kjerstine ; Bengtström, Linda ; Taxvig, Camilla ; Trier, Xenia ; Petersen, Jens Højslev ; Svingen, Terje ; Binderup, Mona-Lise ; van Vugt-Lussenburg, Barbare Medea Akuce ; Dybdahl, Marianne ; Granby, Kit ; Vinggaard, Anne Marie. / An effect-directed strategy for characterizing emerging chemicals in food contact materials made from paper and board. In: Food and Chemical Toxicology. 2017 ; Vol. 106, No. Part A. pp. 250-259.
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abstract = "Food contact materials (FCM) are any type of item intended to come into contact with foods and thus represent a potential source for human exposure to chemicals. Regarding FCMs made of paper and board, information pertaining to their chemical constituents and the potential impacts on human health remains scarce, which hampers safety evaluation. We describe an effect-directed strategy to identify and characterize emerging chemicals in paper and board FCMs. Twenty FCMs were tested in eight reporter gene assays, including assays for the AR, ER, AhR, PPARγ, Nrf2 and p53, as well as mutagenicity. All FCMs exhibited activities in at least one assay. As proof-of-principle, FCM samples obtained from a sandwich wrapper and a pizza box were carried through a complete step-by-step multi-tiered approach. The pizza box exhibited ER activity, likely caused by the presence of bisphenol A, dibutyl phthalate, and benzylbutyl phthalate. The sandwich wrapper exhibited AR antagonism, likely caused by abietic acid and dehydroabietic acid. Migration studies confirmed that the active chemicals can transfer from FCMs to food simulants. In conclusion, we report an effect-directed strategy that can identify hazards posed by FCMs made from paper and board, including the identification of the chemical(s) responsible for the observed activity.",
keywords = "Abietic acid, Bisphenol A, Effect-directed analysis, Food packaging materials, Hazard identification, In vitro tests, Paper and board, Phthalates",
author = "Rosenmai, {Anna Kjerstine} and Linda Bengtstr{\"o}m and Camilla Taxvig and Xenia Trier and Petersen, {Jens H{\o}jslev} and Terje Svingen and Mona-Lise Binderup and {van Vugt-Lussenburg}, {Barbare Medea Akuce} and Marianne Dybdahl and Kit Granby and Vinggaard, {Anne Marie}",
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language = "English",
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pages = "250--259",
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An effect-directed strategy for characterizing emerging chemicals in food contact materials made from paper and board. / Rosenmai, Anna Kjerstine; Bengtström, Linda; Taxvig, Camilla; Trier, Xenia; Petersen, Jens Højslev; Svingen, Terje; Binderup, Mona-Lise; van Vugt-Lussenburg, Barbare Medea Akuce; Dybdahl, Marianne; Granby, Kit; Vinggaard, Anne Marie.

In: Food and Chemical Toxicology, Vol. 106, No. Part A, 2017, p. 250-259.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - An effect-directed strategy for characterizing emerging chemicals in food contact materials made from paper and board

AU - Rosenmai, Anna Kjerstine

AU - Bengtström, Linda

AU - Taxvig, Camilla

AU - Trier, Xenia

AU - Petersen, Jens Højslev

AU - Svingen, Terje

AU - Binderup, Mona-Lise

AU - van Vugt-Lussenburg, Barbare Medea Akuce

AU - Dybdahl, Marianne

AU - Granby, Kit

AU - Vinggaard, Anne Marie

PY - 2017

Y1 - 2017

N2 - Food contact materials (FCM) are any type of item intended to come into contact with foods and thus represent a potential source for human exposure to chemicals. Regarding FCMs made of paper and board, information pertaining to their chemical constituents and the potential impacts on human health remains scarce, which hampers safety evaluation. We describe an effect-directed strategy to identify and characterize emerging chemicals in paper and board FCMs. Twenty FCMs were tested in eight reporter gene assays, including assays for the AR, ER, AhR, PPARγ, Nrf2 and p53, as well as mutagenicity. All FCMs exhibited activities in at least one assay. As proof-of-principle, FCM samples obtained from a sandwich wrapper and a pizza box were carried through a complete step-by-step multi-tiered approach. The pizza box exhibited ER activity, likely caused by the presence of bisphenol A, dibutyl phthalate, and benzylbutyl phthalate. The sandwich wrapper exhibited AR antagonism, likely caused by abietic acid and dehydroabietic acid. Migration studies confirmed that the active chemicals can transfer from FCMs to food simulants. In conclusion, we report an effect-directed strategy that can identify hazards posed by FCMs made from paper and board, including the identification of the chemical(s) responsible for the observed activity.

AB - Food contact materials (FCM) are any type of item intended to come into contact with foods and thus represent a potential source for human exposure to chemicals. Regarding FCMs made of paper and board, information pertaining to their chemical constituents and the potential impacts on human health remains scarce, which hampers safety evaluation. We describe an effect-directed strategy to identify and characterize emerging chemicals in paper and board FCMs. Twenty FCMs were tested in eight reporter gene assays, including assays for the AR, ER, AhR, PPARγ, Nrf2 and p53, as well as mutagenicity. All FCMs exhibited activities in at least one assay. As proof-of-principle, FCM samples obtained from a sandwich wrapper and a pizza box were carried through a complete step-by-step multi-tiered approach. The pizza box exhibited ER activity, likely caused by the presence of bisphenol A, dibutyl phthalate, and benzylbutyl phthalate. The sandwich wrapper exhibited AR antagonism, likely caused by abietic acid and dehydroabietic acid. Migration studies confirmed that the active chemicals can transfer from FCMs to food simulants. In conclusion, we report an effect-directed strategy that can identify hazards posed by FCMs made from paper and board, including the identification of the chemical(s) responsible for the observed activity.

KW - Abietic acid

KW - Bisphenol A

KW - Effect-directed analysis

KW - Food packaging materials

KW - Hazard identification

KW - In vitro tests

KW - Paper and board

KW - Phthalates

U2 - 10.1016/j.fct.2017.05.061

DO - 10.1016/j.fct.2017.05.061

M3 - Journal article

VL - 106

SP - 250

EP - 259

JO - Food and Chemical Toxicology

JF - Food and Chemical Toxicology

SN - 0278-6915

IS - Part A

ER -