An E2-Substituted Chimeric Pestivirus With DIVA Vaccine Properties.

Thomas Bruun Rasmussen, Åse Uttenthal, Jens Nielsen, Ilona Reimann, Sandra Blome, Martin Beer

Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

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Abstract

An advantage of the use of chimeric pestiviruses as modified live vaccines against classical swine fever (CSF) resides in their capacity to be manipulated to achieve the characteristics desired for safe and efficacious DIVA vaccines. We have recently generated a new chimeric virus, Riems26_E2gif engineered specifically for this purpose. The E2-substituted Riems26_E2gif was derived by homologues recombination of the complete E2 protein encoding genome region from Border disease strain Gifhorn into a bacterial artificial chromosome (BAC) harbouring the genome of the CSFV vaccine strain C-Riems. The virulence, immunogenicity and vaccine properties of Riems26_E2gif were tested in a vaccine-challenge experiment in pigs. Riems26_E2gif vaccinated pigs could be differentiated from infected pigs using a CSFV-E2 specific ELISA. Following challenge infection with highly virulent CSFV strain Koslov, all vaccinated pigs were protected. This new chimeric pestivirus represents a C-strain based DIVA vaccine candidate that can be differentiated based on CSFV E2 specific antibodies.
Original languageEnglish
Publication date2011
Publication statusPublished - 2011
Event8th ESVV Pestivirus Symposium - Hannover, Germany
Duration: 25 Sep 201128 Sep 2011
Conference number: 8
http://www.pestivirus2011.de/

Conference

Conference8th ESVV Pestivirus Symposium
Number8
CountryGermany
CityHannover
Period25/09/201128/09/2011
Internet address

Cite this

Rasmussen, T. B., Uttenthal, Å., Nielsen, J., Reimann, I., Blome, S., & Beer, M. (2011). An E2-Substituted Chimeric Pestivirus With DIVA Vaccine Properties.. Abstract from 8th ESVV Pestivirus Symposium, Hannover, Germany.
Rasmussen, Thomas Bruun ; Uttenthal, Åse ; Nielsen, Jens ; Reimann, Ilona ; Blome, Sandra ; Beer, Martin. / An E2-Substituted Chimeric Pestivirus With DIVA Vaccine Properties. Abstract from 8th ESVV Pestivirus Symposium, Hannover, Germany.
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abstract = "An advantage of the use of chimeric pestiviruses as modified live vaccines against classical swine fever (CSF) resides in their capacity to be manipulated to achieve the characteristics desired for safe and efficacious DIVA vaccines. We have recently generated a new chimeric virus, Riems26_E2gif engineered specifically for this purpose. The E2-substituted Riems26_E2gif was derived by homologues recombination of the complete E2 protein encoding genome region from Border disease strain Gifhorn into a bacterial artificial chromosome (BAC) harbouring the genome of the CSFV vaccine strain C-Riems. The virulence, immunogenicity and vaccine properties of Riems26_E2gif were tested in a vaccine-challenge experiment in pigs. Riems26_E2gif vaccinated pigs could be differentiated from infected pigs using a CSFV-E2 specific ELISA. Following challenge infection with highly virulent CSFV strain Koslov, all vaccinated pigs were protected. This new chimeric pestivirus represents a C-strain based DIVA vaccine candidate that can be differentiated based on CSFV E2 specific antibodies.",
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Rasmussen, TB, Uttenthal, Å, Nielsen, J, Reimann, I, Blome, S & Beer, M 2011, 'An E2-Substituted Chimeric Pestivirus With DIVA Vaccine Properties.' 8th ESVV Pestivirus Symposium, Hannover, Germany, 25/09/2011 - 28/09/2011, .

An E2-Substituted Chimeric Pestivirus With DIVA Vaccine Properties. / Rasmussen, Thomas Bruun; Uttenthal, Åse; Nielsen, Jens; Reimann, Ilona; Blome, Sandra; Beer, Martin.

2011. Abstract from 8th ESVV Pestivirus Symposium, Hannover, Germany.

Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

TY - ABST

T1 - An E2-Substituted Chimeric Pestivirus With DIVA Vaccine Properties.

AU - Rasmussen, Thomas Bruun

AU - Uttenthal, Åse

AU - Nielsen, Jens

AU - Reimann, Ilona

AU - Blome, Sandra

AU - Beer, Martin

PY - 2011

Y1 - 2011

N2 - An advantage of the use of chimeric pestiviruses as modified live vaccines against classical swine fever (CSF) resides in their capacity to be manipulated to achieve the characteristics desired for safe and efficacious DIVA vaccines. We have recently generated a new chimeric virus, Riems26_E2gif engineered specifically for this purpose. The E2-substituted Riems26_E2gif was derived by homologues recombination of the complete E2 protein encoding genome region from Border disease strain Gifhorn into a bacterial artificial chromosome (BAC) harbouring the genome of the CSFV vaccine strain C-Riems. The virulence, immunogenicity and vaccine properties of Riems26_E2gif were tested in a vaccine-challenge experiment in pigs. Riems26_E2gif vaccinated pigs could be differentiated from infected pigs using a CSFV-E2 specific ELISA. Following challenge infection with highly virulent CSFV strain Koslov, all vaccinated pigs were protected. This new chimeric pestivirus represents a C-strain based DIVA vaccine candidate that can be differentiated based on CSFV E2 specific antibodies.

AB - An advantage of the use of chimeric pestiviruses as modified live vaccines against classical swine fever (CSF) resides in their capacity to be manipulated to achieve the characteristics desired for safe and efficacious DIVA vaccines. We have recently generated a new chimeric virus, Riems26_E2gif engineered specifically for this purpose. The E2-substituted Riems26_E2gif was derived by homologues recombination of the complete E2 protein encoding genome region from Border disease strain Gifhorn into a bacterial artificial chromosome (BAC) harbouring the genome of the CSFV vaccine strain C-Riems. The virulence, immunogenicity and vaccine properties of Riems26_E2gif were tested in a vaccine-challenge experiment in pigs. Riems26_E2gif vaccinated pigs could be differentiated from infected pigs using a CSFV-E2 specific ELISA. Following challenge infection with highly virulent CSFV strain Koslov, all vaccinated pigs were protected. This new chimeric pestivirus represents a C-strain based DIVA vaccine candidate that can be differentiated based on CSFV E2 specific antibodies.

M3 - Conference abstract for conference

ER -

Rasmussen TB, Uttenthal Å, Nielsen J, Reimann I, Blome S, Beer M. An E2-Substituted Chimeric Pestivirus With DIVA Vaccine Properties.. 2011. Abstract from 8th ESVV Pestivirus Symposium, Hannover, Germany.