Abstract
P>Direct measurements of cells from photo micrographs are becoming increasingly used when investigating the position and/or distribution of chromosomal loci in bacteria. In general, these measurements have been done manually, and without clear definition of how they are made. Here we present a procedure for standardizing the measurement of cell properties from phase contrast images. Furthermore, we present a program using these standardized methods that can measure the intracellular positions of fluorescent foci in bacterial cells faster and with more precision than manual measurement.
Original language | English |
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Journal | Journal of Microscopy |
Volume | 239 |
Issue number | 3 |
Pages (from-to) | 194-199 |
ISSN | 0022-2720 |
DOIs | |
Publication status | Published - 2010 |
Keywords
- Automatic analysis of bacterial properties
- fluorescent chromosome locations
- chromosome segregation