An automated and highly efficient method for counting and measuring fluorescent foci in rod-shaped bacteria

Henrik Jørck Nielsen, Flemming G. Hansen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    P>Direct measurements of cells from photo micrographs are becoming increasingly used when investigating the position and/or distribution of chromosomal loci in bacteria. In general, these measurements have been done manually, and without clear definition of how they are made. Here we present a procedure for standardizing the measurement of cell properties from phase contrast images. Furthermore, we present a program using these standardized methods that can measure the intracellular positions of fluorescent foci in bacterial cells faster and with more precision than manual measurement.
    Original languageEnglish
    JournalJournal of Microscopy
    Volume239
    Issue number3
    Pages (from-to)194-199
    ISSN0022-2720
    DOIs
    Publication statusPublished - 2010

    Keywords

    • Automatic analysis of bacterial properties
    • fluorescent chromosome locations
    • chromosome segregation

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