An autoinducible trp-T7 expression system for production of proteins and biochemicals in Escherichia coli

Jenny Landberg, Hemanshu Mundhada*, Alex Toftgaard Nielsen

*Corresponding author for this work

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Abstract

Inducible expression systems can be applied to control the expression of proteins or biochemical pathways in cell factories. However, several of the established systems require the addition of expensive inducers, making them unfeasible for large-scale production. Here, we establish a genome integrated trp-T7 expression system where tryptophan can be used to control the induction of a gene or a metabolic pathway. We show that the initiation of gene expression from low- and high-copy vectors can be tuned by varying the initial concentration of tryptophan or yeast extract, and that expression is tightly regulated and homogenous when compared with the commonly used lac-T7 system. Finally, we apply the trp-T7 expression system for the production of l-serine, where we reach titers of 26 g/L in fed-batch fermentation.

Original languageEnglish
JournalBiotechnology and Bioengineering
Volume117
Issue number5
Pages (from-to)1513-1524
ISSN0006-3592
DOIs
Publication statusPublished - 2020

Keywords

  • Escherichia coli
  • inducible promoter
  • l-serine
  • tryptophan

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