Amplified suspension magnetic bead-based assay for sensitive detection of anti-glycan antibodies as potential cancer biomarkers

Anna Blsakova, Filip Květoň, Lenka Lorencová, Ola Blixt, Alica Vikartovska, Peter Kasak*, Jan Tkac*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

The development of a novel SUspension Magnetic-Bead-based Assay (SUMBA) for the detection of antibodies against aberrant glycans (AGA) as potential cancer biomarkers is presented here. The SUMBA method was extensively optimised by choosing proper commercially available AGA able to specifically, and with high affinity, recognise aberrant glycans, which were attached to the protein backbone working as a molecular scaffold (a glycoconjugate). The whole SUMBA was optimised using several analytical techniques such as Surface Plasmon Resonance and Energy Dispersive X-ray Spectroscopy. Additionally, the SUMBA method was extensively optimised for signal enhancement. With all steps optimised, we were able to detect AGA ultrasensitively with a limit of detection of 0.45 pM. Moreover, AGA could be detected in serum samples with a recovery index in the range of 98%–104%.
Original languageEnglish
Article number339444
JournalAnalytica Chimica Acta
Volume1195
Number of pages9
ISSN0003-2670
DOIs
Publication statusPublished - 2022

Keywords

  • Aberrant glycans
  • Antibodies against aberrant glycans
  • Magnetic separation
  • Suspension assay

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