Amplified fragment length polymorphism and pulsed field gel electrophoresis for subspecies differentiation of Serpulina pilosicoli

Kristian Møller, Tim Kåre Jensen, Mette Boye, T.D. Leser, Peter Ahrens

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    Abstract

    Pulsed field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP) were compared for their ability to differentiate between 50 porcine Serpulina pilosicoli isolates. Both techniques were highly sensitive, dividing the isolates into 36 and 38 groups, respectively. Due to the low workload and high capacity and sensitivity of AFLP, this technique is recommended for future studies of S. pilosicoli. S. pilosicoli isolates were genetically diverse, and generally identical subtypes were only identified in cultures from the same herd.
    Original languageEnglish
    JournalAnaerobe
    Volume5
    Issue number3-4
    Pages (from-to)313-315
    ISSN1075-9964
    DOIs
    Publication statusPublished - 1999

    Keywords

    • subtypes
    • AFLP
    • serpulina pilosicoli
    • PFGE

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