Ampicillin-Improved Glucose Tolerance in Diet-Induced Obese C57BL/6NTac Mice Is Age Dependent

Research output: Contribution to journalJournal article – Annual report year: 2014Researchpeer-review

  • Author: Rune, Ida

    University of Copenhagen, Denmark

  • Author: Hansen, C. H. F.

    University of Copenhagen

  • Author: Ellekilde, Merete

    University of Copenhagen, Denmark

  • Author: Nielsen, D. S.

    University of Copenhagen, Denmark

  • Author: Skovgaard, Kerstin

    Section for Immunology and Vaccinology, National Veterinary Institute, Technical University of Denmark, Kemitorvet, 2800, Kgs. Lyngby, Denmark

  • Author: Rolin, B. C.

    Novo Nordisk A/S, Denmark

  • Author: Lykkesfeldt, J.

    University of Copenhagen, Denmark

  • Author: Josefsen, K.

    Copenhagen University Hospital, Denmark

  • Author: Tranberg, Britt

    University of Copenhagen, Denmark

  • Author: Kihl, P.

    University of Copenhagen, Denmark

  • Author: Hansen, A. K.

    University of Copenhagen

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Ampicillin has been shown to improve glucose tolerance in mice. We hypothesized that this effect is present only if treatment is initiated prior to weaning and that it disappears when treatment is terminated. High-fat fed C57BL/6NTac mice were divided into groups that received Ampicillin at different ages or not at all. We found that both diet and Ampicillin significantly changed the gut microbiota composition in the animals. Furthermore, there was a significant improvement in glucose tolerance in Ampicillin-treated, five-week-old mice compared to nontreated mice in the control group. At study termination, expressions of mRNA coding for tumor necrosis factor, serum amyloid A, and lactase were upregulated, while the expression of tumor necrosis factor (ligand) superfamily member 15 was downregulated in the ileum of Ampicillin-treated mice. Higher dendritic cell percentages were found systemically in high-fat diet mice, and a lower tolerogenic dendritic cell percentage was found both in relation to high-fat diet and late Ampicillin treatment. The results support our hypothesis that a "window" exists early in life in which an alteration of the gut microbiota affects glucose tolerance as well as development of gut immunity and that this window may disappear after weaning.
Original languageEnglish
Article number319321
JournalJournal of Diabetes Research
Volume2013
Number of pages13
ISSN2314-6745
DOIs
Publication statusPublished - 2013

Bibliographical note

Copyright © 2013 I. Rune et al.This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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