TY - JOUR
T1 - Amperometric monitoring of redox activity in living yeast cells: comparison of menadione and menadione sodium bisulfite as electron transfer mediators
AU - Heiskanen, Arto
AU - Yakovleva, Julia
AU - Spégel, Christer F.
AU - Taboryski, Rafael J.
AU - Koudelka-Hep, Milena
AU - Emnéus, Jenny
AU - Ruzgas, Tautgirdas
PY - 2004
Y1 - 2004
N2 - An amperometric method was applied for real-time monitoring of intracellular redox enzyme activity. Baker's yeast (Saccharomyces cerevisiae) cells were immobilized on platinum microband electrodes and mediated anodic currents were measured. The currents were observed in the absence and in the presence of glucose as a source of reducing equivalents, NADH and NADPH. 2-Methyl-1,4-naphthoquinone (menadione, vitamin K-3) and water soluble 2-methyl-1,4-naphthoquinone sodium bisulfite (menadione sodium bisulfite MSB) were compared as artificial electron acceptors for their ability to transduce internal cellular redox activity into electrode current. It was found that hydrophobic menadione was superior to its water-soluble bisulfite derivative for probing whole intact cells. (C) 2003 Elsevier B.V. All rights reserved.
AB - An amperometric method was applied for real-time monitoring of intracellular redox enzyme activity. Baker's yeast (Saccharomyces cerevisiae) cells were immobilized on platinum microband electrodes and mediated anodic currents were measured. The currents were observed in the absence and in the presence of glucose as a source of reducing equivalents, NADH and NADPH. 2-Methyl-1,4-naphthoquinone (menadione, vitamin K-3) and water soluble 2-methyl-1,4-naphthoquinone sodium bisulfite (menadione sodium bisulfite MSB) were compared as artificial electron acceptors for their ability to transduce internal cellular redox activity into electrode current. It was found that hydrophobic menadione was superior to its water-soluble bisulfite derivative for probing whole intact cells. (C) 2003 Elsevier B.V. All rights reserved.
U2 - 10.1016/j.elecom.2003.12.003
DO - 10.1016/j.elecom.2003.12.003
M3 - Journal article
SN - 1388-2481
VL - 6
SP - 219
EP - 224
JO - Electrochemistry Communications
JF - Electrochemistry Communications
IS - 2
ER -