Ammonia-induced inhibition of manure-based continuous biomethanation process under different organic loading rates and associated microbial community dynamics

M. L. Christou, S. Vasileiadis, S. D. Kalamaras, D. G. Karpouzas, Irini Angelidaki, T. A. Kotsopoulos*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Three Continuously Stirred Tank Reactors (CTSRs) were operating at steady state conditions with Organic Loading Rates (OLR) of 2.09, 3.024 and 4.0 g VS L−1 d−1. Glucose was used as the sole factor for increasing the OLR, linking the increase of the OLR with the C/N ratio increase. The reactors were stressed by increasing the ammonia concentration to 5 g L−1 from 1.862 g L−1. The results showed elevating inhibition of the anaerobic process by increasing the C/N ratio just by increasing the OLR, under the high ammonia concentration. A different response of the bacterial and archaeal community under ammonia stressed conditions was also observed. Under the high ammonia concentration, hydrogen-depended methylotrophic was the dominant methanogenesis route at OLR of 2.09 g VS L−1d−1, while the hydrogenotrophic route was the dominant at the high OLR of 4 g VS L−1d−1, which coincided with high acetate and propionate concentrations.

Original languageEnglish
Article number124323
JournalBioresource Technology
Volume320
ISSN0960-8524
DOIs
Publication statusPublished - 2021

Keywords

  • Ammonia inhibition
  • Anaerobic digestion
  • Microbial community
  • OLR

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