Ammonia effect on hydrogenotrophic methanogens and syntrophic acetate oxidizing bacteria

Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

418 Downloads (Pure)


Substrates that contain high ammonia levels can cause inhibition on anaerobic digestion process and unstable biogas production. The aim of the current study was to assess the effects of different ammonia levels on pure strains of (syntrophic acetate oxidizing) SAO bacteria and hydrogenotrophic methanogens. Two pure strains of hydrogenotrophic methanogens (i.e: Methanoculleus bourgensis and Methanoculleus thermophiles) and two pure strains of SAO bacteria (i.e: Tepidanaerobacter acetatoxydans and Thermacetogenium phaeum) were inoculated under four different ammonia (0.26, 3, 5 and 7g NH4+-N/L) and free ammonia levels (Mesophilic: 3.31, 38.2, 63.68 and 89.15 g NH3-N/L. Thermophilic: 8.48, 97.82, 163.03 and 228.24 g NH3-N/L). The results indicated that both T. acetatoxydans and T. phaeum were more sensitive to high ammonia levels compared to the hydrogenotrophic methanogens tested. Additionally, the total incubation periods of hydrogenotrophic methanogens were significantly shorter compared to the SAO bacteria incubation periods. Thus, it seems that hydrogenotrophic methanogens could be equally, if not more, tolerant to high ammonia levels compared to SAO bacteria.
Original languageEnglish
Publication date2015
Number of pages4
Publication statusPublished - 2015
Event14th World Congress of Anaerobic Digestion - , Chile
Duration: 14 Nov 201518 Nov 2015
Conference number: 14


Conference14th World Congress of Anaerobic Digestion


  • Ammonia inhibition
  • Anaerobic digestion
  • Biogas Hydrogenotrophic methanogens
  • SAO bacteria


Dive into the research topics of 'Ammonia effect on hydrogenotrophic methanogens and syntrophic acetate oxidizing bacteria'. Together they form a unique fingerprint.

Cite this