All-polymer biosensor for label-free point of care diagnostics

Research output: Book/ReportPh.D. thesis – Annual report year: 2013Research

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All-polymer biosensor for label-free point of care diagnostics. / Dapra, Johannes.

2013. 121 p.

Research output: Book/ReportPh.D. thesis – Annual report year: 2013Research

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@phdthesis{2b3ccc2d44a042e089f9e60107743baa,
title = "All-polymer biosensor for label-free point of care diagnostics",
abstract = "Personalised medicine and diagnostics is a rapidly growing field of research and general interest. Important tools for individual patient care are so called point-of-care devices. These typically simple and inexpensive instruments allow the untrained user to perform simple diagnostic analyses without the need for a specialised laboratory. Other fields of application are for example health care projects in developing countries where access to modern high-throughput facilities is often impossible or sectors not related to the medical field, like environmental monitoring or food safety. The aim of this PhD project was to develop a modular platform based on electrochemical impedimetric sensing. This device can easily be modified by changing the biological receptors and therefore offers a broad range of possible applications. To keep the costs and the environmental footprint low the entire biosensor was designed in plastic; featuring a microfluidic channel and an electrode system fabricated from conductive polymers. Aptamers were used as recognition elements providing a more stable alternative to antibodies for easier handling and a longer shelf life. Moreover, aptamers have a much wider range of possible target molecules than antibodies. The biosensor platform was successfully adapted to different tasks and tested against three very different analytes: DNA, antibiotics and virus particles. Throughout the experiments the sensors showed high sensitivity and were able to detect very low analyte concentrations in both buffered solutions, milk and saliva samples.",
author = "Johannes Dapra",
year = "2013",
language = "English",

}

RIS

TY - BOOK

T1 - All-polymer biosensor for label-free point of care diagnostics

AU - Dapra, Johannes

PY - 2013

Y1 - 2013

N2 - Personalised medicine and diagnostics is a rapidly growing field of research and general interest. Important tools for individual patient care are so called point-of-care devices. These typically simple and inexpensive instruments allow the untrained user to perform simple diagnostic analyses without the need for a specialised laboratory. Other fields of application are for example health care projects in developing countries where access to modern high-throughput facilities is often impossible or sectors not related to the medical field, like environmental monitoring or food safety. The aim of this PhD project was to develop a modular platform based on electrochemical impedimetric sensing. This device can easily be modified by changing the biological receptors and therefore offers a broad range of possible applications. To keep the costs and the environmental footprint low the entire biosensor was designed in plastic; featuring a microfluidic channel and an electrode system fabricated from conductive polymers. Aptamers were used as recognition elements providing a more stable alternative to antibodies for easier handling and a longer shelf life. Moreover, aptamers have a much wider range of possible target molecules than antibodies. The biosensor platform was successfully adapted to different tasks and tested against three very different analytes: DNA, antibiotics and virus particles. Throughout the experiments the sensors showed high sensitivity and were able to detect very low analyte concentrations in both buffered solutions, milk and saliva samples.

AB - Personalised medicine and diagnostics is a rapidly growing field of research and general interest. Important tools for individual patient care are so called point-of-care devices. These typically simple and inexpensive instruments allow the untrained user to perform simple diagnostic analyses without the need for a specialised laboratory. Other fields of application are for example health care projects in developing countries where access to modern high-throughput facilities is often impossible or sectors not related to the medical field, like environmental monitoring or food safety. The aim of this PhD project was to develop a modular platform based on electrochemical impedimetric sensing. This device can easily be modified by changing the biological receptors and therefore offers a broad range of possible applications. To keep the costs and the environmental footprint low the entire biosensor was designed in plastic; featuring a microfluidic channel and an electrode system fabricated from conductive polymers. Aptamers were used as recognition elements providing a more stable alternative to antibodies for easier handling and a longer shelf life. Moreover, aptamers have a much wider range of possible target molecules than antibodies. The biosensor platform was successfully adapted to different tasks and tested against three very different analytes: DNA, antibiotics and virus particles. Throughout the experiments the sensors showed high sensitivity and were able to detect very low analyte concentrations in both buffered solutions, milk and saliva samples.

M3 - Ph.D. thesis

BT - All-polymer biosensor for label-free point of care diagnostics

ER -