Alleviation of glucose repression of maltose metabolism by MIG1 disruption in Saccharomyces cerevisiae

Christopher Klein, Lisbeth Olsson, B. Rønnow, J.D. Mikkelsen, Jens Bredal Nielsen

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    Abstract

    The MIG1 gene was disrupted in a haploid laboratory strain (B224) and in an industrial polyploid strain (DGI 342) of Saccharomyces cerevisiae. The alleviation of glucose repression of the expression of MAL genes and alleviation of glucose control of maltose metabolism were investigated in batch cultivations on glucose-maltose mixtures. In the MIG1-disrupted haploid strain, glucose repression was partly alleviated; i.e., maltose metabolism was initiated at higher glucose concentrations than in the corresponding wild-type strain. In contrast, the polyploid Delta mig1 strain exhibited an even more stringent glucose control of maltose metabolism than the corresponding wild-type strain, which could be explained by a more rigid catabolite inactivation of maltose permease, affecting the uptake of maltose. Growth on the glucose-sucrose mixture showed that the polyploid Delta mig1 strain was relieved of glucose repression of the SUC genes. The disruption of MIG1 was shown to bring about pleiotropic effects, manifested in changes in the pattern of secreted metabolites and in the specific growth rate.
    Original languageEnglish
    JournalApplied and Environmental Microbiology
    Volume62
    Issue number12
    Pages (from-to)4441-4449
    ISSN0099-2240
    Publication statusPublished - 1996

    Cite this

    Klein, C., Olsson, L., Rønnow, B., Mikkelsen, J. D., & Nielsen, J. B. (1996). Alleviation of glucose repression of maltose metabolism by MIG1 disruption in Saccharomyces cerevisiae. Applied and Environmental Microbiology, 62(12), 4441-4449.