All-in-one disulfide bridging enables the generation of antibody conjugates with modular cargo loading

Friederike M. Dannheim, Stephen J. Walsh, Carolina T. Orozco, Anders Højgaard Hansen, Jonathan D. Bargh, Sophie E. Jackson, Nicholas J. Bond, Jeremy S. Parker, Jason S. Carroll*, David R. Spring*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Antibody-drug conjugates (ADCs) are valuable therapeutic entities which leverage the specificity of antibodies to selectively deliver cytotoxins to antigen-expressing targets such as cancer cells. However, current methods for their construction still suffer from a number of shortcomings. For instance, using a single modification technology to modulate the drug-to-antibody ratio (DAR) in integer increments while maintaining homogeneity and stability remains exceptionally challenging. Herein, we report a novel method for the generation of antibody conjugates with modular cargo loading from native antibodies. Our approach relies on a new class of disulfide rebridging linkers, which can react with eight cysteine residues, thereby effecting all-in-one bridging of all four interchain disulfides in an IgG1 antibody with a single linker molecule. Modification of the antibody with the linker in a 1 : 1 ratio enabled the modulation of cargo loading in a quick and selective manner through derivatization of the linker with varying numbers of payload attachment handles to allow for attachment of either 1, 2, 3 or 4 payloads (fluorescent dyes or cytotoxins). Assessment of the biological activity of these conjugates demonstrated their exceptional stability in human plasma and utility for cell-selective cytotoxin delivery or imaging/diagnostic applications.
Original languageEnglish
Article number8781
JournalChemical Science
Volume13
Pages (from-to)8781-8790
Number of pages10
ISSN2041-6520
DOIs
Publication statusPublished - 2022

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