All-fibre supercontinuum laser for in vivo multispectral photoacoustic microscopy of lipids in the extended near-infrared region

Manoj Kumar Dasa, Gianni Nteroli, Patrick Bowen, Giulia Messa, Yuyang Feng, Christian Rosenberg Petersen, Stella Koutsikou, Magalie Bondu, Peter M. Moselund, Adrian Podoleanu, Adrian Bradu, Christos Markos, Ole Bang

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Among the numerous endogenous biological molecules, information on lipids is highly coveted for understanding both aspects of developmental biology and research in fatal chronic diseases. Due to the pronounced absorption features of lipids in the extended near-infrared region (1650−1850 nm), visualisation and identification of lipids become possible using multi-spectral photoacoustic (optoacoustic) microscopy. However, the spectroscopic studies in this spectral region require lasers that can produce high pulse energies over a broad spectral bandwidth to efficiently excite strong photoacoustic signals. The most well-known laser sources capable of satisfying the multi-spectral photoacoustic microscopy requirements (tunability and pulse energy) are tunable nanosecond optical parametric oscillators. However, these lasers have an inherently large footprint, thus preventing their use in compact microscopy systems. Besides, they exhibit low-repetition rates. Here, we demonstrate a compact all-fibre, high pulse energy supercontinuum laser that covers a spectral range from 1440 to 1870 nm with a 7 ns pulse duration and total energy of 18.3 μJ at a repetition rate of 100 kHz. Using the developed high-pulse energy source, we perform multi-spectral photoacoustic microscopy imaging of lipids, both ex vivo on adipose tissue and in vivo to study the development of Xenopus laevis tadpoles, using six different excitation bands over the first overtone transition of C–H vibration bonds (1650−1850 nm).
Original languageEnglish
Article number100163
JournalPhotoacoustics
Volume18
Number of pages7
ISSN2213-5979
DOIs
Publication statusPublished - 2020

Keywords

  • Photoacoustic microscopy
  • Fibre lasers
  • Supercontinuum
  • Lipids

Cite this

Dasa, M. K., Nteroli, G., Bowen, P., Messa, G., Feng, Y., Petersen, C. R., Koutsikou, S., Bondu, M., Moselund, P. M., Podoleanu, A., Bradu, A., Markos, C., & Bang, O. (2020). All-fibre supercontinuum laser for in vivo multispectral photoacoustic microscopy of lipids in the extended near-infrared region. Photoacoustics, 18, [100163]. https://doi.org/10.1016/j.pacs.2020.100163