Advantages of Foxp3+ regulatory T cell depletion using DEREG mice: Advantages of Foxp3+regulatory T cell depletion

Christian T Mayer, Katharina Lahl, Pedro Milanez-Almeida, Deepika Watts, Ulf Dittmer, Nanna Fyhrquist, Jochen Huehn, Manfred Kopf, Karsten Kretschmer, Barry Rouse, Tim Sparwasser

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Abstract

Several mechanisms enable immunological self-tolerance. Regulatory T cells (Tregs) are a specialized T cell subset that prevents autoimmunity and excessive immune responses, but can also mediate detrimental tolerance to tumors and pathogens in a Foxp3-dependent manner. Genetic tools exploiting the foxp3 locus including bacterial artificial chromosome (BAC)-transgenic DEREG mice have provided essential information on Treg biology and the potential therapeutic modulation of tolerance. In DEREG mice, Foxp3(+) Tregs selectively express eGFP and diphtheria toxin (DT) receptor, allowing for the specific depletion of Tregs through DT administration. We here provide a detailed overview about important considerations such as DT toxicity, which affects any mouse strain treated with DT, and Treg rebound after depletion. Additionally, we point out the specific advantages of BAC-transgenic DEREG mice including their suitability to study organ-specific autoimmunity such as type I diabetes. Moreover, we discuss recent insights into the role of Tregs in viral infections. In summary, DEREG mice are an important tool to study Treg-mediated tolerance and its therapeutic circumvention.
Original languageEnglish
JournalImmunity, Inflammation and Disease
Volume2
Issue number3
Pages (from-to)162-165
Number of pages4
ISSN2050-4527
DOIs
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • Autoimmunity
  • DEREG
  • Treg
  • diphtheria toxin (DT)
  • regulatory T cells
  • tolerance

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