Advances in synthetic biology of oleaginous yeast Yarrowia lipolytica for producing non-native chemicals

Research output: Contribution to journalJournal article – Annual report year: 2018Researchpeer-review

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Advances in synthetic biology of oleaginous yeast Yarrowia lipolytica for producing non-native chemicals. / Darvishi, Farshad; Ariana, Mehdi; Marella, Eko Roy; Borodina, Irina.

In: Applied Microbiology and Biotechnology, Vol. 102, No. 14, 2018, p. 5925-5938.

Research output: Contribution to journalJournal article – Annual report year: 2018Researchpeer-review

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@article{2acc5907a15d4f8ba861cbda69c46662,
title = "Advances in synthetic biology of oleaginous yeast Yarrowia lipolytica for producing non-native chemicals",
abstract = "Oleaginous yeast Yarrowia lipolytica is an important industrial host for the production of enzymes, oils, fragrances, surfactants, cosmetics, and pharmaceuticals. More recently, improved synthetic biology tools have allowed more extensive engineering of this yeast species, which lead to the production of non-native metabolites. In this review, we summarize the recent advances of genome editing tools for Y. lipolytica, including the application of CRISPR/Cas9 system and discuss case studies, where Y. lipolytica was engineered to produce various non-native chemicals: short-chain fatty alcohols and alkanes as biofuels, polyunsaturated fatty acids for nutritional and pharmaceutical applications, polyhydroxyalkanoates and dicarboxylic acids as precursors for biodegradable plastics, carotenoid-type pigments for food and feed, and campesterol as a precursor for steroid drugs.",
keywords = "Bio-based chemicals, CRISPR/Cas9, Synthetic biology, Yarrowia lipolytica",
author = "Farshad Darvishi and Mehdi Ariana and Marella, {Eko Roy} and Irina Borodina",
year = "2018",
doi = "10.1007/s00253-018-9099-x",
language = "English",
volume = "102",
pages = "5925--5938",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer",
number = "14",

}

RIS

TY - JOUR

T1 - Advances in synthetic biology of oleaginous yeast Yarrowia lipolytica for producing non-native chemicals

AU - Darvishi, Farshad

AU - Ariana, Mehdi

AU - Marella, Eko Roy

AU - Borodina, Irina

PY - 2018

Y1 - 2018

N2 - Oleaginous yeast Yarrowia lipolytica is an important industrial host for the production of enzymes, oils, fragrances, surfactants, cosmetics, and pharmaceuticals. More recently, improved synthetic biology tools have allowed more extensive engineering of this yeast species, which lead to the production of non-native metabolites. In this review, we summarize the recent advances of genome editing tools for Y. lipolytica, including the application of CRISPR/Cas9 system and discuss case studies, where Y. lipolytica was engineered to produce various non-native chemicals: short-chain fatty alcohols and alkanes as biofuels, polyunsaturated fatty acids for nutritional and pharmaceutical applications, polyhydroxyalkanoates and dicarboxylic acids as precursors for biodegradable plastics, carotenoid-type pigments for food and feed, and campesterol as a precursor for steroid drugs.

AB - Oleaginous yeast Yarrowia lipolytica is an important industrial host for the production of enzymes, oils, fragrances, surfactants, cosmetics, and pharmaceuticals. More recently, improved synthetic biology tools have allowed more extensive engineering of this yeast species, which lead to the production of non-native metabolites. In this review, we summarize the recent advances of genome editing tools for Y. lipolytica, including the application of CRISPR/Cas9 system and discuss case studies, where Y. lipolytica was engineered to produce various non-native chemicals: short-chain fatty alcohols and alkanes as biofuels, polyunsaturated fatty acids for nutritional and pharmaceutical applications, polyhydroxyalkanoates and dicarboxylic acids as precursors for biodegradable plastics, carotenoid-type pigments for food and feed, and campesterol as a precursor for steroid drugs.

KW - Bio-based chemicals

KW - CRISPR/Cas9

KW - Synthetic biology

KW - Yarrowia lipolytica

U2 - 10.1007/s00253-018-9099-x

DO - 10.1007/s00253-018-9099-x

M3 - Journal article

VL - 102

SP - 5925

EP - 5938

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 14

ER -