Advances in synthetic biology of oleaginous yeast Yarrowia lipolytica for producing non-native chemicals

Farshad Darvishi*, Mehdi Ariana, Eko Roy Marella, Irina Borodina

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Oleaginous yeast Yarrowia lipolytica is an important industrial host for the production of enzymes, oils, fragrances, surfactants, cosmetics, and pharmaceuticals. More recently, improved synthetic biology tools have allowed more extensive engineering of this yeast species, which lead to the production of non-native metabolites. In this review, we summarize the recent advances of genome editing tools for Y. lipolytica, including the application of CRISPR/Cas9 system and discuss case studies, where Y. lipolytica was engineered to produce various non-native chemicals: short-chain fatty alcohols and alkanes as biofuels, polyunsaturated fatty acids for nutritional and pharmaceutical applications, polyhydroxyalkanoates and dicarboxylic acids as precursors for biodegradable plastics, carotenoid-type pigments for food and feed, and campesterol as a precursor for steroid drugs.
Original languageEnglish
JournalApplied Microbiology and Biotechnology
Volume102
Issue number14
Pages (from-to)5925-5938
ISSN0175-7598
DOIs
Publication statusPublished - 2018

Keywords

  • Bio-based chemicals
  • CRISPR/Cas9
  • Synthetic biology
  • Yarrowia lipolytica

Fingerprint Dive into the research topics of 'Advances in synthetic biology of oleaginous yeast Yarrowia lipolytica for producing non-native chemicals'. Together they form a unique fingerprint.

Cite this