TY - JOUR
T1 - Advances in antibody phage display technology
AU - Ledsgaard, Line
AU - Ljungars, Anne
AU - Rimbault, Charlotte
AU - Sørensen, Christoffer V.
AU - Tulika, Tulika
AU - Wade, Jack
AU - Wouters, Yessica
AU - McCafferty, John
AU - Laustsen, Andreas H.
PY - 2022
Y1 - 2022
N2 - Phage display technology can be used for the discovery of antibodies for research, diagnostic, and therapeutic purposes. In this review, we present and discuss key parameters that can be optimized when performing phage display selection campaigns, including the use of different antibody formats and advanced strategies for antigen presentation, such as immobilization, liposomes, nanodiscs, virus-like particles, and whole cells. Furthermore, we provide insights into selection strategies that can be used for the discovery of antibodies with complex binding requirements, such as targeting a specific epitope, cross-reactivity, or pH-dependent binding. Lastly, we provide a description of specialized phage display libraries for the discovery of bispecific antibodies and pH-sensitive antibodies. Together, these methods can be used to improve antibody discovery campaigns against all types of antigen.
AB - Phage display technology can be used for the discovery of antibodies for research, diagnostic, and therapeutic purposes. In this review, we present and discuss key parameters that can be optimized when performing phage display selection campaigns, including the use of different antibody formats and advanced strategies for antigen presentation, such as immobilization, liposomes, nanodiscs, virus-like particles, and whole cells. Furthermore, we provide insights into selection strategies that can be used for the discovery of antibodies with complex binding requirements, such as targeting a specific epitope, cross-reactivity, or pH-dependent binding. Lastly, we provide a description of specialized phage display libraries for the discovery of bispecific antibodies and pH-sensitive antibodies. Together, these methods can be used to improve antibody discovery campaigns against all types of antigen.
KW - Phage display
KW - Antibody discovery
KW - Antigen presentation
KW - Selection strategy
KW - Library design
U2 - 10.1016/j.drudis.2022.05.002
DO - 10.1016/j.drudis.2022.05.002
M3 - Review
C2 - 35550436
VL - 27
SP - 2151
EP - 2169
JO - Drug Discovery Today
JF - Drug Discovery Today
SN - 1359-6446
IS - 8
ER -