Advances in antibody phage display technology

Line Ledsgaard; Anne Ljungars; Charlotte Rimbault; Christoffer V. Sørensen; Tulika Tulika; Jack Wade; Yessica Wouters; John McCafferty; Andreas H. Laustsen

doi:10.1016/j.drudis.2022.05.002

Advances in antibody phage display technology

Line Ledsgaard^*, Anne Ljungars, Charlotte Rimbault, Christoffer V. Sørensen, Tulika Tulika, Jack Wade, Yessica Wouters, John McCafferty, Andreas H. Laustsen^*

^*Corresponding author for this work

University of Cambridge

Research output: Contribution to journal › Review › peer-review

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Abstract

Phage display technology can be used for the discovery of antibodies for research, diagnostic, and therapeutic purposes. In this review, we present and discuss key parameters that can be optimized when performing phage display selection campaigns, including the use of different antibody formats and advanced strategies for antigen presentation, such as immobilization, liposomes, nanodiscs, virus-like particles, and whole cells. Furthermore, we provide insights into selection strategies that can be used for the discovery of antibodies with complex binding requirements, such as targeting a specific epitope, cross-reactivity, or pH-dependent binding. Lastly, we provide a description of specialized phage display libraries for the discovery of bispecific antibodies and pH-sensitive antibodies. Together, these methods can be used to improve antibody discovery campaigns against all types of antigen.

Original language	English
Journal	Drug Discovery Today
Volume	27
Issue number	8
Pages (from-to)	2151-2169
Number of pages	19
ISSN	1359-6446
DOIs	https://doi.org/10.1016/j.drudis.2022.05.002
Publication status	Published - 2022

Keywords

Phage display
Antibody discovery
Antigen presentation
Selection strategy
Library design

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AU - Ledsgaard, Line

AU - Ljungars, Anne

AU - Rimbault, Charlotte

AU - Sørensen, Christoffer V.

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AU - Wade, Jack

AU - Wouters, Yessica

AU - McCafferty, John

AU - Laustsen, Andreas H.

PY - 2022

Y1 - 2022

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AB - Phage display technology can be used for the discovery of antibodies for research, diagnostic, and therapeutic purposes. In this review, we present and discuss key parameters that can be optimized when performing phage display selection campaigns, including the use of different antibody formats and advanced strategies for antigen presentation, such as immobilization, liposomes, nanodiscs, virus-like particles, and whole cells. Furthermore, we provide insights into selection strategies that can be used for the discovery of antibodies with complex binding requirements, such as targeting a specific epitope, cross-reactivity, or pH-dependent binding. Lastly, we provide a description of specialized phage display libraries for the discovery of bispecific antibodies and pH-sensitive antibodies. Together, these methods can be used to improve antibody discovery campaigns against all types of antigen.

KW - Phage display

KW - Antibody discovery

KW - Antigen presentation

KW - Selection strategy

KW - Library design

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DO - 10.1016/j.drudis.2022.05.002

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Advances in antibody phage display technology

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