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Advanced Microscopy of Microbial Cells

    Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

    Abstract

    Growing awareness of heterogeneity in cells of microbial populations has emphasized the importance of advanced microscopy for visualization and understanding of the molecular mechanisms underlying cell-to-cell variation. In this review, we highlight some of the recent advances in confocal microscopy, super-resolution optical microscopy (STED, SIM, PALM) as well as atomic force microscopy and Raman spectroscopy. Using examples of bistability in microbial populations as well as biofilm development and differentiation in bacterial and yeast consortia, we demonstrate the importance of microscopy for visualization of variation between cells in phenotypic traits such as gene expression.
    Original languageEnglish
    Title of host publicationAdvances in Biochemical Engineering/Biotechnology
    Volume124
    PublisherSpringer
    Publication date2011
    Pages21-54
    ISBN (Print)978-3-642-16886-4
    ISBN (Electronic)978-3-642-16887-1
    Publication statusPublished - 2011
    SeriesAdvances in Biochemical Engineering/Biotechnology
    ISSN0724-6145

    Keywords

    • Bacteria
    • Bistability
    • Advanced microscopy techniques
    • Single-cell gene expression
    • Biofilm development and differentiation
    • Yeast

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