Advanced fluidic handling and use of two-phase flow for high throughput structural investigation of proteins on a microfluidic sample preparation platform

Josiane P. Lafleur, Detlef Snakenborg, M. Møller, K. N. Toft, J. K. Jacobsen, B. Vestergaard, L. Arleth, Jörg Peter Kutter

    Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

    Abstract

    Research on the structure of proteins can bring forth a wealth of information about biological function and can be used to better understand the processes in living cells. This paper reports a new microfluidic sample preparation system for the structural investigation of proteins by Small Angle X-ray Scattering (SAXS). The system includes hardware and software features for precise fluidic control, synchrotron beamline control, UV absorbance measurements and automated data analysis. The precise fluidic handling capabilities are used to transport and precisely position samples as small as 500 nL into the microfluidic chip’s detection chambers.
    Original languageEnglish
    Title of host publicationProceedings of MicroTAS 2010
    Publication date2010
    ISBN (Print)978-0-9798064-3-8
    Publication statusPublished - 2010
    Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Groningen, Netherlands
    Duration: 3 Oct 20107 Oct 2010
    Conference number: 14

    Conference

    Conference14th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    Number14
    CountryNetherlands
    CityGroningen
    Period03/10/201007/10/2010

    Keywords

    • Sample preparation
    • Small Angle X-ray Scattering
    • Two-phase flow
    • Protein analysis

    Fingerprint

    Dive into the research topics of 'Advanced fluidic handling and use of two-phase flow for high throughput structural investigation of proteins on a microfluidic sample preparation platform'. Together they form a unique fingerprint.

    Cite this