Adult Neural Stem Cells and Multiciliated Ependymal Cells Share a Common Lineage Regulated by the Geminin Family Members

Gonzalo Ortiz-Alvarez; Marie Daclin; ASM Shihavuddin; Pauline Lansade; Aurelien Fortoul; Solene Clavreul; Maria-Eleni Lalioti; Stavros Taraviras; Simon Hippenmeyer; Jean Livet; Alice Meunier; Auguste Genovesio; Nathalie Spassky

doi:10.1016/j.neuron.2019.01.051

Adult Neural Stem Cells and Multiciliated Ependymal Cells Share a Common Lineage Regulated by the Geminin Family Members

Gonzalo Ortiz-Alvarez, Marie Daclin, ASM Shihavuddin, Pauline Lansade, Aurelien Fortoul, Solene Clavreul, Maria-Eleni Lalioti, Stavros Taraviras, Simon Hippenmeyer, Jean Livet, Alice Meunier, Auguste Genovesio, Nathalie Spassky^*

^*Corresponding author for this work

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Abstract

Adult neural stem cells and multiciliated ependymal cells are glial cells essential for neurological functions. Together, they make up the adult neurogenic niche. Using both high-throughput clonal analysis and single-cell resolution of progenitor division patterns and fate, we show that these two components of the neurogenic niche are lineally related: adult neural stem cells are sister cells to ependymal cells, whereas most ependymal cells arise from the terminal symmetric divisions of the lineage. Unexpectedly, we found that the antagonist regulators of DNA replication, GemC1 and Geminin, can tune the proportion of neural stem cells and ependymal cells. Our findings reveal the controlled dynamic of the neurogenic niche ontogeny and identify the Geminin family members as key regulators of the initial pool of adult
neural stem cells.

Original language	English
Journal	Neuron
Volume	102
Issue number	1
Pages (from-to)	159-172
ISSN	0896-6273
DOIs	https://doi.org/10.1016/j.neuron.2019.01.051
Publication status	Published - 2019

Keywords

Neural stem cells
Multiciliated ependymal cells
Radial glial cells
Cell lineage
Symmetric division
Geminin family
GemC1
Multiciliogenesis
Cell division

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Ortiz-Alvarez, G., Daclin, M., Shihavuddin, ASM., Lansade, P., Fortoul, A., Clavreul, S., Lalioti, M.-E., Taraviras, S., Hippenmeyer, S., Livet, J., Meunier, A., Genovesio, A., & Spassky, N. (2019). Adult Neural Stem Cells and Multiciliated Ependymal Cells Share a Common Lineage Regulated by the Geminin Family Members. Neuron, 102(1), 159-172. https://doi.org/10.1016/j.neuron.2019.01.051

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abstract = "Adult neural stem cells and multiciliated ependymal cells are glial cells essential for neurological functions. Together, they make up the adult neurogenic niche. Using both high-throughput clonal analysis and single-cell resolution of progenitor division patterns and fate, we show that these two components of the neurogenic niche are lineally related: adult neural stem cells are sister cells to ependymal cells, whereas most ependymal cells arise from the terminal symmetric divisions of the lineage. Unexpectedly, we found that the antagonist regulators of DNA replication, GemC1 and Geminin, can tune the proportion of neural stem cells and ependymal cells. Our findings reveal the controlled dynamic of the neurogenic niche ontogeny and identify the Geminin family members as key regulators of the initial pool of adultneural stem cells.",

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author = "Gonzalo Ortiz-Alvarez and Marie Daclin and ASM Shihavuddin and Pauline Lansade and Aurelien Fortoul and Solene Clavreul and Maria-Eleni Lalioti and Stavros Taraviras and Simon Hippenmeyer and Jean Livet and Alice Meunier and Auguste Genovesio and Nathalie Spassky",

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Ortiz-Alvarez, G, Daclin, M, Shihavuddin, ASM, Lansade, P, Fortoul, A, Clavreul, S, Lalioti, M-E, Taraviras, S, Hippenmeyer, S, Livet, J, Meunier, A, Genovesio, A & Spassky, N 2019, 'Adult Neural Stem Cells and Multiciliated Ependymal Cells Share a Common Lineage Regulated by the Geminin Family Members', Neuron, vol. 102, no. 1, pp. 159-172. https://doi.org/10.1016/j.neuron.2019.01.051

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T1 - Adult Neural Stem Cells and Multiciliated Ependymal Cells Share a Common Lineage Regulated by the Geminin Family Members

AU - Ortiz-Alvarez, Gonzalo

AU - Daclin, Marie

AU - Shihavuddin, ASM

AU - Lansade, Pauline

AU - Fortoul, Aurelien

AU - Clavreul, Solene

AU - Lalioti, Maria-Eleni

AU - Taraviras, Stavros

AU - Hippenmeyer, Simon

AU - Livet, Jean

AU - Meunier, Alice

AU - Genovesio, Auguste

AU - Spassky, Nathalie

PY - 2019

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N2 - Adult neural stem cells and multiciliated ependymal cells are glial cells essential for neurological functions. Together, they make up the adult neurogenic niche. Using both high-throughput clonal analysis and single-cell resolution of progenitor division patterns and fate, we show that these two components of the neurogenic niche are lineally related: adult neural stem cells are sister cells to ependymal cells, whereas most ependymal cells arise from the terminal symmetric divisions of the lineage. Unexpectedly, we found that the antagonist regulators of DNA replication, GemC1 and Geminin, can tune the proportion of neural stem cells and ependymal cells. Our findings reveal the controlled dynamic of the neurogenic niche ontogeny and identify the Geminin family members as key regulators of the initial pool of adultneural stem cells.

AB - Adult neural stem cells and multiciliated ependymal cells are glial cells essential for neurological functions. Together, they make up the adult neurogenic niche. Using both high-throughput clonal analysis and single-cell resolution of progenitor division patterns and fate, we show that these two components of the neurogenic niche are lineally related: adult neural stem cells are sister cells to ependymal cells, whereas most ependymal cells arise from the terminal symmetric divisions of the lineage. Unexpectedly, we found that the antagonist regulators of DNA replication, GemC1 and Geminin, can tune the proportion of neural stem cells and ependymal cells. Our findings reveal the controlled dynamic of the neurogenic niche ontogeny and identify the Geminin family members as key regulators of the initial pool of adultneural stem cells.

KW - Neural stem cells

KW - Multiciliated ependymal cells

KW - Radial glial cells

KW - Cell lineage

KW - Symmetric division

KW - Geminin family

KW - GemC1

KW - Multiciliogenesis

KW - Cell division

U2 - 10.1016/j.neuron.2019.01.051

DO - 10.1016/j.neuron.2019.01.051

M3 - Journal article

C2 - 30824354

SN - 0896-6273

VL - 102

SP - 159

EP - 172

JO - Neuron

JF - Neuron

IS - 1

ER -

Adult Neural Stem Cells and Multiciliated Ependymal Cells Share a Common Lineage Regulated by the Geminin Family Members

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Keywords

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