Adsorption and protein-induced metal release from chromium metal and stainless steel

M. Lundin, Y. Hedberg, T. Jiang, G. Herting, X. Wang, Esben Thormann, E. Blomberg, I. Odnevall Wallinder

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A research effort is undertaken to understand the mechanism of metal release from, e.g., inhaled metal particles or metal implants in the presence of proteins. The effect of protein adsorption on the metal release process from oxidized chromium metal surfaces and stainless steel surfaces was therefore examined by quartz crystal microbalance with energy dissipation monitoring (QCM-D) and graphite furnace atomic absorption spectroscopy (GFAAS). Differently charged and sized proteins, relevant for the inhalation and dermal exposure route were chosen including human and bovine serum albumin (HSA, BSA), mucin (BSM), and lysozyme (LYS). The results show that all proteins have high affinities for chromium and stainless steel (AISI 316) when deposited from solutions at pH 4 and at pH 7.4 where the protein adsorbed amount was very similar. Adsorption of albumin and mucin was substantially higher at pH 4 compared to pH 7.4 with approximately monolayer coverage at pH 7.4, whereas lysozyme adsorbed in multilayers at both investigated pH. The protein–surface interaction was strong since proteins were irreversibly adsorbed with respect to rinsing. Due to the passive nature of chromium and stainless steel (AISI 316) surfaces, very low metal release concentrations from the QCM metal surfaces in the presence of proteins were obtained on the time scale of the adsorption experiment. Therefore, metal release studies from massive metal sheets in contact with protein solutions were carried out in parallel. The presence of proteins increased the extent of metals released for chromium metal and stainless steel grades of different microstructure and alloy content, all with passive chromium(III)-rich surface oxides, such as QCM (AISI 316), ferritic (AISI 430), austentic (AISI 304, 316L), and duplex (LDX 2205).
Original languageEnglish
JournalJournal of Colloid and Interface Science
Volume366
Issue number1
Pages (from-to)155-164
ISSN0021-9797
DOIs
Publication statusPublished - 2012
Externally publishedYes

Keywords

  • Chromium
  • Protein adsorption
  • Metal release
  • Stainless steel
  • Lysozyme
  • Albumin
  • Mucin

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