Additional diterpenes from Physcomitrella patens synthesized by copalyl diphosphate/kaurene synthase (PpCPS/KS)

Xin Zhan, Søren Spanner Bach, Nikolaj Lervad Hansen, Christina Lunde, Henrik Toft Simonsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The bifunctional diterpene synthase, copalyl diphosphate/kaurene synthase from the moss Physcomitrella patens (PpCPS/KS), catalyses the formation of at least four diterpenes, including ent-beyerene, ent-sandaracopimaradiene, ent-kaur-16-ene, and 16-hydroxy-ent-kaurene. The enzymatic activity has been confirmed through generation of a targeted PpCPS/KS knock-out mutant in P. patens via homologous recombination, through transient expression of PpCPS/KS in Nicotiana benthamiana, and expression of PpCPS/KS in E. coli. GC-MS analysis of the knock-out mutant shows that it lacks the diterpenoids, supporting that all are products of PpCPS/KS as observed in N. benthamiana and E. coli. These results provide additional knowledge of the mechanism of this bifunctional diterpene synthase, and are in line with proposed reaction mechanisms in kaurene biosynthesis.
Original languageEnglish
JournalPlant Physiology and Biochemistry
Volume96
Pages (from-to)110-114
Number of pages5
ISSN0981-9428
DOIs
Publication statusPublished - 2015
Externally publishedYes

Keywords

  • Diterpene
  • Copalyl diphosphate/kaurene synthase
  • Physcomitrella patens
  • Ent-beyerene
  • Ent-sandaracopimaradiene

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