Adaption of Saccharomyces cerevisiae expressing a heterologous protein

Astrid Mørkeberg Krogh, Vibe Beck, Lars Højlund Christensen, Claus Maxel Henriksen, Kasper Møller, Lisbeth Olsson

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Production of the heterologous protein, bovine aprotinin, in Saccharomyces cerevisiae was shown to affect the metabolism of the host cell to various extent depending on the strain genotype. Strains with different genotypes, industrial and laboroatory, respectively, were investigated. The maximal specific growth rate of the strains was reduced by 54% and 33%, respectively, upon the introduction of the gene encoding aprotinin. Growing the strains in sequential shake flask cultivations for 250 generations led to an increased maximal specific growth rate and a decrease in the yield of aprotinin as a result of the adaptation. Determination of the level of mRNA encoding aprotinin and the plasmid copy number pointed to different mechanisms responsible for the decline in aprotinin yield in the different strains. (C) 2008 Elsevier B.V. All rights reserved.
    Original languageEnglish
    JournalJournal of Biotechnology
    Volume137
    Issue number1-4
    Pages (from-to)28-33
    ISSN0168-1656
    DOIs
    Publication statusPublished - 2008

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