Active and Passive Immunization Against Staphylococcus aureus Periprosthetic Osteomyelitis in Rats

Niels H. Soe, Nina Vendel Jensen, Asger Lundorff Jensen, Janne Koch, Steen Seier Poulsen, Gerald B. Pier, Helle Krogh Johansen

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Abstract

Background/Aim: Staphylococcus aureus infection associated with orthopedic implants cannot always be controlled. We used a knee prosthesis model with implant-related osteomyelitis in rats to explore induction of an effective immune response with active and passive immunization. Materials and Methods: Fifty-two Sprague-Dawley rats were divided into active (N=28) and passive immunization groups (N=24). A bacterial inoculum of 10(3) S. aureus MN8 was injected into the tibia and the femur marrow before insertion of a non-constrained knee prosthesis in each rat. The active-immunization group received a synthetic oligosaccharide of polysaccharide poly-N-acetylglucosamine (PNAG), 9G1cNH(2) and the passive-immunization group received immunization with immunoglobulin from rabbits infected with S. aureus. Results/Conclusion: Active immunization against PNAG significantly reduced the consequences of osteomyelitis infection from PNAG-producing intercellular adhesion (ica(+)) but not ica(-) S. aureus. Passive immunization resulted in better clinical assessments in animals challenged with either ica(+) or icaS. aureus, suggesting a lack of specificity in this antiserum.
Original languageEnglish
JournalIn Vivo
Volume31
Issue number1
Pages (from-to)45-50
ISSN0258-851X
DOIs
Publication statusPublished - 2017

Bibliographical note

This article is freely accessible online.

Keywords

  • Osteomyelitis
  • Implant-related infection Staphylococcus aureus
  • Immunization
  • Vaccine
  • Ica+ strain
  • Ica− strain

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