Actinobacillus pleuropneumoniae osteomyelitis in pigs demonstrated by fluorescent in situ hybridization

Tim Kåre Jensen, Mette Boye, T. Hagedorn-Olsen, H.J. Riising, Øystein Angen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Necrotizing osteomyelitis and fibrinopurulent arthritis with isolation of Actinobacillus pleuropneumoniae serotype 2 is reported in two pigs from a herd with lameness and mild coughing problems among 8 to 12-week-old pigs. Application of fluorescent in situ hybridization targeting 16S ribosomal RNA of A. pleuropneumoniae in formalin-fixed tissue was performed to verify the association of A. pleuropneumoniae with the bone and joint lesions. By in situ hybridization A. pleuropneumoniae was demonstrated as multiple microcolonies or single cells dispersed in focal fibrinonecrotizing pleuropneumonia, in joints with arthritis, and in bone necroses including lysis of growth plate and suppurative inflammation in the adjacent trabecular metaphysis, thus demonstrating that well-known infections manifest new, unusual lesions.
    Original languageEnglish
    JournalVeterinary Pathology
    Volume36
    Issue number3
    Pages (from-to)258-261
    ISSN0300-9858
    DOIs
    Publication statusPublished - 1999

    Keywords

    • Actinobacillus pleuropneumoniae
    • pigs
    • arthritis
    • in situ hybridization
    • osteomyelitis

    Fingerprint

    Dive into the research topics of 'Actinobacillus pleuropneumoniae osteomyelitis in pigs demonstrated by fluorescent in situ hybridization'. Together they form a unique fingerprint.

    Cite this