Actin filament guidance on a chip: Toward high-throughput assays and lab-on-a-chip applications

Mark Sundberg, Richard Bunk, Nuria Albet-Torres, Anders Kvennefors, Karl Fredrik Persson, Lars Montelius, Ian A. Nicholls, Sara Ghatnekar-Nilsson, Pär Omling, Sven Tågeud, Alf Månsson

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Biological molecular motors that are constrained so that function is effectively limited to predefined nanosized tracks may be used as molecular shuttles in nanotechnological applications. For these applications and in high-throughput functional assays (e.g., drug screening), it is important that the motors propel their cytoskeletal filaments unidirectionally along the tracks with a minimal number of escape events. We here analyze the requirements for achieving this for actin filaments that are propelled by myosin II motor fragments (heavy meromyosin; HMM). First, we tested the guidance of HMM-propelled actin filaments along chemically defined borders. Here, trimethylchlorosilane (TMCS)-derivatized areas with high-quality HMM function were surrounded by SiO2 domains where HMM did not bind actin. Guidance along the TMCS-SiO2 border was almost 100% for filament approach angles between 0 and 20 but only about 10% at approach angles near 90. A model (Clemmens, J.; Hess, H.; Lipscomb, R.; Hanein, Y.; Bohringer, K. F.; Matzke, C. M.; Bachand, G. D.; Bunker, B. C.; Vogel, V. Langmuir 2003, 19, 10967-10974) accounted for essential aspects of the data and also correctly predicted a more efficient guidance of actin filaments than previously shown for kinesin-propelled microtubules. Despite the efficient guidance at low approach angles, nanosized (
Original languageEnglish
JournalLangmuir
Volume22
Issue number17
Pages (from-to)7286-7295
ISSN0743-7463
DOIs
Publication statusPublished - 2006
Externally publishedYes

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