Acoustophoretic Synchronization of Mammalian Cells in Microchannels

P. Thévoz, J.D. Adams, H. Shea, Henrik Bruus, H.T. Soh

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    We report the first use of ultrasonic standing waves to achieve cell cycle phase synchronization in mammalian cells in a high-throughput and reagent-free manner. The acoustophoretic cell synchronization (ACS) device utilizes volume-dependent acoustic radiation force within a microchannel to selectively purify target cells of desired phase from an asynchronous mixture based on cell cycle-dependent fluctuations in size. We show that ultrasonic separation allows for gentle, scalable, and label-free synchronization with high G1 phase synchrony (84%) and throughput (3 × 106 cells/h per microchannel).
    Original languageEnglish
    JournalAnalytical Chemistry
    Volume82
    Issue number7
    Pages (from-to)3094-3098
    ISSN0003-2700
    DOIs
    Publication statusPublished - 2010

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