We report the first use of ultrasonic standing waves to achieve cell cycle phase synchronization in mammalian cells in a high-throughput and reagent-free manner. The acoustophoretic cell synchronization (ACS) device utilizes volume-dependent acoustic radiation force within a microchannel to selectively purify target cells of desired phase from an asynchronous mixture based on cell cycle-dependent fluctuations in size. We show that ultrasonic separation allows for gentle, scalable, and label-free synchronization with high G1 phase synchrony (84%) and throughput (3 × 106 cells/h per microchannel).
Thévoz, P., Adams, J. D., Shea, H., Bruus, H., & Soh, H. T. (2010). Acoustophoretic Synchronization of Mammalian Cells in Microchannels. Analytical Chemistry, 82(7), 3094-3098. https://doi.org/10.1021/ac100357u