Acoustic tethering of microorganisms

M. Rode, A. Bioue, F. Miano, H. Bruus, T. Kiørboe, A. Andersen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

We show how to construct and apply a setup to acoustically tether and enable behavioral observations of individual microorganisms using simple laboratory equipment and a standard light microscope. We explore the capability of the setup with the freely swimming dinoflagellate Alexandrium minutum as study organism. We demonstrate that the setup allows us to tether cells in focus in the mid-plane of the sample chamber and make observations of individual organisms at high magnification without affecting their flagellar beat frequencies. We discuss the prospect of the method to explore appendage motion and swimming kinematics of other flagellates and ciliates.
Original languageEnglish
Article numberjeb244089
JournalJournal of Experimental Biology
Volume225
Issue number20
ISSN0022-0949
DOIs
Publication statusPublished - 2022

Keywords

  • Ultrasound
  • Video-microscopy
  • Dinoflagellates

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