Acids ‘generally recognized as safe’ affect morphology and biocompatibility of electrospun chitosan/polyethylene nanofibers

Mai Bay Stie, Megan Jones, Henning Osholm Sørensen, Jette Jacobsen, Ioannis S. Chronakis, Hanne Mørck Nielsen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Electrospinning of neat chitosan is currently achieved by using strong acids or organic solvents, which limits the use of chitosan nanofibers as biocompatible scaffolds for drug delivery and tissue engineering. The aim was to elucidate the effect of specific acids generally recognized as safe (GRAS) on the properties of electrospun chitosan-based nanofibers. Electrospinning chitosan in dilute acetic acid or succinic acid with polyethylene oxide resulted in white and separated nanofibers, whereas nanofibers electrospun in dilute citric acid were transparent and interconnected. Including succinic or citric acid in the spinning process induced disintegration of the fiber mat after four hrs in water, and a concentration-dependent effect on epithelial cell viability. Chitosan nanofibers electrospun in acetic acid maintained their shape and fibrous structure after four hrs in water, and showed no effect on cell viability. This study demonstrates that the choice of GRAS acid highly determines the properties of electrospun chitosan nanofibers.
Original languageEnglish
JournalCarbohydrate Polymers
Volume215
Pages (from-to)253-262
Number of pages10
ISSN0144-8617
DOIs
Publication statusPublished - 2019

Keywords

  • Chitosan
  • Electrospinning
  • Nanofiber properties
  • Solvent
  • Generally recognized as safe

Cite this

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title = "Acids ‘generally recognized as safe’ affect morphology and biocompatibility of electrospun chitosan/polyethylene nanofibers",
abstract = "Electrospinning of neat chitosan is currently achieved by using strong acids or organic solvents, which limits the use of chitosan nanofibers as biocompatible scaffolds for drug delivery and tissue engineering. The aim was to elucidate the effect of specific acids generally recognized as safe (GRAS) on the properties of electrospun chitosan-based nanofibers. Electrospinning chitosan in dilute acetic acid or succinic acid with polyethylene oxide resulted in white and separated nanofibers, whereas nanofibers electrospun in dilute citric acid were transparent and interconnected. Including succinic or citric acid in the spinning process induced disintegration of the fiber mat after four hrs in water, and a concentration-dependent effect on epithelial cell viability. Chitosan nanofibers electrospun in acetic acid maintained their shape and fibrous structure after four hrs in water, and showed no effect on cell viability. This study demonstrates that the choice of GRAS acid highly determines the properties of electrospun chitosan nanofibers.",
keywords = "Chitosan, Electrospinning, Nanofiber properties, Solvent, Generally recognized as safe",
author = "Stie, {Mai Bay} and Megan Jones and S{\o}rensen, {Henning Osholm} and Jette Jacobsen and Chronakis, {Ioannis S.} and Nielsen, {Hanne M{\o}rck}",
year = "2019",
doi = "10.1016/j.carbpol.2019.03.061",
language = "English",
volume = "215",
pages = "253--262",
journal = "Carbohydrate Polymers",
issn = "0144-8617",
publisher = "Pergamon Press",

}

Acids ‘generally recognized as safe’ affect morphology and biocompatibility of electrospun chitosan/polyethylene nanofibers. / Stie, Mai Bay; Jones, Megan; Sørensen, Henning Osholm; Jacobsen, Jette; Chronakis, Ioannis S.; Nielsen, Hanne Mørck.

In: Carbohydrate Polymers, Vol. 215, 2019, p. 253-262.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Acids ‘generally recognized as safe’ affect morphology and biocompatibility of electrospun chitosan/polyethylene nanofibers

AU - Stie, Mai Bay

AU - Jones, Megan

AU - Sørensen, Henning Osholm

AU - Jacobsen, Jette

AU - Chronakis, Ioannis S.

AU - Nielsen, Hanne Mørck

PY - 2019

Y1 - 2019

N2 - Electrospinning of neat chitosan is currently achieved by using strong acids or organic solvents, which limits the use of chitosan nanofibers as biocompatible scaffolds for drug delivery and tissue engineering. The aim was to elucidate the effect of specific acids generally recognized as safe (GRAS) on the properties of electrospun chitosan-based nanofibers. Electrospinning chitosan in dilute acetic acid or succinic acid with polyethylene oxide resulted in white and separated nanofibers, whereas nanofibers electrospun in dilute citric acid were transparent and interconnected. Including succinic or citric acid in the spinning process induced disintegration of the fiber mat after four hrs in water, and a concentration-dependent effect on epithelial cell viability. Chitosan nanofibers electrospun in acetic acid maintained their shape and fibrous structure after four hrs in water, and showed no effect on cell viability. This study demonstrates that the choice of GRAS acid highly determines the properties of electrospun chitosan nanofibers.

AB - Electrospinning of neat chitosan is currently achieved by using strong acids or organic solvents, which limits the use of chitosan nanofibers as biocompatible scaffolds for drug delivery and tissue engineering. The aim was to elucidate the effect of specific acids generally recognized as safe (GRAS) on the properties of electrospun chitosan-based nanofibers. Electrospinning chitosan in dilute acetic acid or succinic acid with polyethylene oxide resulted in white and separated nanofibers, whereas nanofibers electrospun in dilute citric acid were transparent and interconnected. Including succinic or citric acid in the spinning process induced disintegration of the fiber mat after four hrs in water, and a concentration-dependent effect on epithelial cell viability. Chitosan nanofibers electrospun in acetic acid maintained their shape and fibrous structure after four hrs in water, and showed no effect on cell viability. This study demonstrates that the choice of GRAS acid highly determines the properties of electrospun chitosan nanofibers.

KW - Chitosan

KW - Electrospinning

KW - Nanofiber properties

KW - Solvent

KW - Generally recognized as safe

U2 - 10.1016/j.carbpol.2019.03.061

DO - 10.1016/j.carbpol.2019.03.061

M3 - Journal article

VL - 215

SP - 253

EP - 262

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

ER -