TY - JOUR
T1 - Accumulation of magnetic iron oxide nanoparticles coated with variably sized polyethylene glycol in murine tumors
AU - Larsen, Esben Kjær Unmack
AU - Nielsen, Thomas
AU - Wittenborn, Thomas
AU - Rydtoft, Louise Munk
AU - Lokanathan, Arcot R.
AU - Hansen, Line
AU - Ostergaard, Leif
AU - Kingshott, Peter
AU - Howard, Kenneth Alan
AU - Besenbacher, Flemming
AU - Nielsen, Niels Chr.
AU - Kjems, Jørgen
PY - 2012
Y1 - 2012
N2 - Iron oxide nanoparticles have found widespread applications in different areas including cell separation, drug delivery and as contrast agents. Due to water insolubility and stability issues, nanoparticles utilized for biological applications require coatings such as the commonly employed polyethylene glycol (PEG). Despite its frequent use, the influence of PEG coatings on the physicochemical and biological properties of iron nanoparticles has hitherto not been studied in detail. To address this, we studied the effect of 333-20000 Da PEG coatings that resulted in larger hydrodynamic size, lower surface charge, longer circulation half-life, and lower uptake in macrophage cells when the particles were coated with high molecular weight (Mw) PEG molecules. By use of magnetic resonance imaging, we show coating-dependent in vivo uptake in murine tumors with an optimal coating Mw of 10000 Da. © 2012 The Royal Society of Chemistry.
AB - Iron oxide nanoparticles have found widespread applications in different areas including cell separation, drug delivery and as contrast agents. Due to water insolubility and stability issues, nanoparticles utilized for biological applications require coatings such as the commonly employed polyethylene glycol (PEG). Despite its frequent use, the influence of PEG coatings on the physicochemical and biological properties of iron nanoparticles has hitherto not been studied in detail. To address this, we studied the effect of 333-20000 Da PEG coatings that resulted in larger hydrodynamic size, lower surface charge, longer circulation half-life, and lower uptake in macrophage cells when the particles were coated with high molecular weight (Mw) PEG molecules. By use of magnetic resonance imaging, we show coating-dependent in vivo uptake in murine tumors with an optimal coating Mw of 10000 Da. © 2012 The Royal Society of Chemistry.
KW - Drug delivery
KW - Magnetic resonance imaging
KW - Metal nanoparticles
KW - Polyethylene glycols
KW - Tumors
KW - Coatings
U2 - 10.1039/c2nr11554a
DO - 10.1039/c2nr11554a
M3 - Journal article
C2 - 22395568
SN - 2040-3364
VL - 4
SP - 2352
EP - 2361
JO - Nanoscale
JF - Nanoscale
IS - 7
ER -