Acclimatization contributes to stable anaerobic digestion of organic fraction of municipal solid waste under extreme ammonia levels: Focusing on microbial community dynamics

Miao Yan, Ioannis Fotidis*, Hailin Tian, Benyamin Khoshnevisan, Laura Treu, Panagiotis Tsapekos, Irini Angelidaki

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The organic fraction of municipal solid waste (OFMSW) is an abundant and sustainable substrate for the anaerobic digestion (AD) process, yet ammonia released during OFMSW hydrolysis could result in suboptimal biogas production. Acclimatized ammonia tolerant microorganisms offer an efficient way to alleviate ammonia inhibition during AD. This study aimed to achieve an efficient AD of OFMSW under extreme ammonia levels and elucidate the dynamics of the acclimatized microbial community. Thus, two mesophilic continuous stirred tank reactors (CSTR), fed only with OFMSW, were successfully acclimatized up to 8.5 g NH4+-N/L, and their methane yields fluctuated <10%, compared to the methane yields without ammonia addition. Microbiological analyses showed that Methanosaeta concilii and Methanosarcina soligelidi were the dominant methanogens at low and high ammonia levels, respectively. Whilst, a unique metabolic pathway shift, from aceticlastic to hydrogenotrophic methanogenesis, of M. soligelidi was identified during the acclimatization process.
Original languageEnglish
Article number121376
JournalBioresource Technology
Volume286
Number of pages7
ISSN0960-8524
DOIs
Publication statusPublished - 2019

Keywords

  • Ammonia acclimatization
  • Hydrogenotrophic pathway
  • Aceticlastic pathway
  • Metabolic pathway shift
  • Methanosarcina soligelidi

Cite this

@article{23725e902e47418490379f6f4047f86f,
title = "Acclimatization contributes to stable anaerobic digestion of organic fraction of municipal solid waste under extreme ammonia levels: Focusing on microbial community dynamics",
abstract = "The organic fraction of municipal solid waste (OFMSW) is an abundant and sustainable substrate for the anaerobic digestion (AD) process, yet ammonia released during OFMSW hydrolysis could result in suboptimal biogas production. Acclimatized ammonia tolerant microorganisms offer an efficient way to alleviate ammonia inhibition during AD. This study aimed to achieve an efficient AD of OFMSW under extreme ammonia levels and elucidate the dynamics of the acclimatized microbial community. Thus, two mesophilic continuous stirred tank reactors (CSTR), fed only with OFMSW, were successfully acclimatized up to 8.5 g NH4+-N/L, and their methane yields fluctuated <10{\%}, compared to the methane yields without ammonia addition. Microbiological analyses showed that Methanosaeta concilii and Methanosarcina soligelidi were the dominant methanogens at low and high ammonia levels, respectively. Whilst, a unique metabolic pathway shift, from aceticlastic to hydrogenotrophic methanogenesis, of M. soligelidi was identified during the acclimatization process.",
keywords = "Ammonia acclimatization, Hydrogenotrophic pathway, Aceticlastic pathway, Metabolic pathway shift, Methanosarcina soligelidi",
author = "Miao Yan and Ioannis Fotidis and Hailin Tian and Benyamin Khoshnevisan and Laura Treu and Panagiotis Tsapekos and Irini Angelidaki",
year = "2019",
doi = "10.1016/j.biortech.2019.121376",
language = "English",
volume = "286",
journal = "Bioresource Technology",
issn = "0960-8524",
publisher = "Elsevier",

}

TY - JOUR

T1 - Acclimatization contributes to stable anaerobic digestion of organic fraction of municipal solid waste under extreme ammonia levels: Focusing on microbial community dynamics

AU - Yan, Miao

AU - Fotidis, Ioannis

AU - Tian, Hailin

AU - Khoshnevisan, Benyamin

AU - Treu, Laura

AU - Tsapekos, Panagiotis

AU - Angelidaki, Irini

PY - 2019

Y1 - 2019

N2 - The organic fraction of municipal solid waste (OFMSW) is an abundant and sustainable substrate for the anaerobic digestion (AD) process, yet ammonia released during OFMSW hydrolysis could result in suboptimal biogas production. Acclimatized ammonia tolerant microorganisms offer an efficient way to alleviate ammonia inhibition during AD. This study aimed to achieve an efficient AD of OFMSW under extreme ammonia levels and elucidate the dynamics of the acclimatized microbial community. Thus, two mesophilic continuous stirred tank reactors (CSTR), fed only with OFMSW, were successfully acclimatized up to 8.5 g NH4+-N/L, and their methane yields fluctuated <10%, compared to the methane yields without ammonia addition. Microbiological analyses showed that Methanosaeta concilii and Methanosarcina soligelidi were the dominant methanogens at low and high ammonia levels, respectively. Whilst, a unique metabolic pathway shift, from aceticlastic to hydrogenotrophic methanogenesis, of M. soligelidi was identified during the acclimatization process.

AB - The organic fraction of municipal solid waste (OFMSW) is an abundant and sustainable substrate for the anaerobic digestion (AD) process, yet ammonia released during OFMSW hydrolysis could result in suboptimal biogas production. Acclimatized ammonia tolerant microorganisms offer an efficient way to alleviate ammonia inhibition during AD. This study aimed to achieve an efficient AD of OFMSW under extreme ammonia levels and elucidate the dynamics of the acclimatized microbial community. Thus, two mesophilic continuous stirred tank reactors (CSTR), fed only with OFMSW, were successfully acclimatized up to 8.5 g NH4+-N/L, and their methane yields fluctuated <10%, compared to the methane yields without ammonia addition. Microbiological analyses showed that Methanosaeta concilii and Methanosarcina soligelidi were the dominant methanogens at low and high ammonia levels, respectively. Whilst, a unique metabolic pathway shift, from aceticlastic to hydrogenotrophic methanogenesis, of M. soligelidi was identified during the acclimatization process.

KW - Ammonia acclimatization

KW - Hydrogenotrophic pathway

KW - Aceticlastic pathway

KW - Metabolic pathway shift

KW - Methanosarcina soligelidi

U2 - 10.1016/j.biortech.2019.121376

DO - 10.1016/j.biortech.2019.121376

M3 - Journal article

VL - 286

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

M1 - 121376

ER -