TY - JOUR
T1 - Acclimatization contributes to stable anaerobic digestion of organic fraction of municipal solid waste under extreme ammonia levels: Focusing on microbial community dynamics
AU - Yan, Miao
AU - Fotidis, Ioannis
AU - Tian, Hailin
AU - Khoshnevisan, Benyamin
AU - Treu, Laura
AU - Tsapekos, Panagiotis
AU - Angelidaki, Irini
PY - 2019
Y1 - 2019
N2 - The organic fraction of municipal solid waste (OFMSW) is an abundant and sustainable substrate for the anaerobic digestion (AD) process, yet ammonia released during OFMSW hydrolysis could result in suboptimal biogas production. Acclimatized ammonia tolerant microorganisms offer an efficient way to alleviate ammonia inhibition during AD. This study aimed to achieve an efficient AD of OFMSW under extreme ammonia levels and elucidate the dynamics of the acclimatized microbial community. Thus, two mesophilic continuous stirred tank reactors (CSTR), fed only with OFMSW, were successfully acclimatized up to 8.5 g NH4+-N/L, and their methane yields fluctuated <10%, compared to the methane yields without ammonia addition. Microbiological analyses showed that Methanosaeta concilii and Methanosarcina soligelidi were the dominant methanogens at low and high ammonia levels, respectively. Whilst, a unique metabolic pathway shift, from aceticlastic to hydrogenotrophic methanogenesis, of M. soligelidi was identified during the acclimatization process.
AB - The organic fraction of municipal solid waste (OFMSW) is an abundant and sustainable substrate for the anaerobic digestion (AD) process, yet ammonia released during OFMSW hydrolysis could result in suboptimal biogas production. Acclimatized ammonia tolerant microorganisms offer an efficient way to alleviate ammonia inhibition during AD. This study aimed to achieve an efficient AD of OFMSW under extreme ammonia levels and elucidate the dynamics of the acclimatized microbial community. Thus, two mesophilic continuous stirred tank reactors (CSTR), fed only with OFMSW, were successfully acclimatized up to 8.5 g NH4+-N/L, and their methane yields fluctuated <10%, compared to the methane yields without ammonia addition. Microbiological analyses showed that Methanosaeta concilii and Methanosarcina soligelidi were the dominant methanogens at low and high ammonia levels, respectively. Whilst, a unique metabolic pathway shift, from aceticlastic to hydrogenotrophic methanogenesis, of M. soligelidi was identified during the acclimatization process.
KW - Ammonia acclimatization
KW - Hydrogenotrophic pathway
KW - Aceticlastic pathway
KW - Metabolic pathway shift
KW - Methanosarcina soligelidi
U2 - 10.1016/j.biortech.2019.121376
DO - 10.1016/j.biortech.2019.121376
M3 - Journal article
C2 - 31030070
SN - 0960-8524
VL - 286
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 121376
ER -