Acclimation to extremely high ammonia levels in continuous biomethanation process and the associated microbial community dynamics

Hailin Tian, Ioannis Fotidis*, Enrico Mancini, Laura Treu, Ahmed Mahdy, Mercedes Ballesteros, Cristina González-Fernández, Irini Angelidaki

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Acclimatized anaerobic communities to high ammonia levels can offer a solution to the ammonia toxicity problem in biogas reactors. In the current study, a stepwise acclimation strategy up to 10 g NH4+-N L−1, was performed in mesophilic (37 ± 1 °C) continuously stirred tank reactors. The reactors were co-digesting (20/80 based on volatile solid) cattle slurry and microalgae, a protein-rich, 3rd generation biomass. Throughout the acclimation period, methane production was stable with more than 95% of the uninhibited yield. Next generation 16S rRNA gene sequencing revealed a dramatic microbiome change throughout the ammonia acclimation process. Clostridium ultunense, a syntrophic acetate oxidizing bacteria, increased significantly alongside with hydrogenotrophic methanogen Methanoculleus spp., indicating strong hydrogenotrophic methanogenic activity at extreme ammonia levels (>7 g NH4+-N L−1). Overall, this study demonstrated for the first time that acclimation of methanogenic communities to extreme ammonia levels in continuous AD process is possible, by developing a specialised acclimation AD microbiome.
Original languageEnglish
JournalBioresource Technology
Volume247
Pages (from-to)616-623
ISSN0960-8524
DOIs
Publication statusPublished - 2018

Cite this

Tian, Hailin ; Fotidis, Ioannis ; Mancini, Enrico ; Treu, Laura ; Mahdy, Ahmed ; Ballesteros, Mercedes ; González-Fernández, Cristina ; Angelidaki, Irini. / Acclimation to extremely high ammonia levels in continuous biomethanation process and the associated microbial community dynamics. In: Bioresource Technology. 2018 ; Vol. 247. pp. 616-623.
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title = "Acclimation to extremely high ammonia levels in continuous biomethanation process and the associated microbial community dynamics",
abstract = "Acclimatized anaerobic communities to high ammonia levels can offer a solution to the ammonia toxicity problem in biogas reactors. In the current study, a stepwise acclimation strategy up to 10 g NH4+-N L−1, was performed in mesophilic (37 ± 1 °C) continuously stirred tank reactors. The reactors were co-digesting (20/80 based on volatile solid) cattle slurry and microalgae, a protein-rich, 3rd generation biomass. Throughout the acclimation period, methane production was stable with more than 95{\%} of the uninhibited yield. Next generation 16S rRNA gene sequencing revealed a dramatic microbiome change throughout the ammonia acclimation process. Clostridium ultunense, a syntrophic acetate oxidizing bacteria, increased significantly alongside with hydrogenotrophic methanogen Methanoculleus spp., indicating strong hydrogenotrophic methanogenic activity at extreme ammonia levels (>7 g NH4+-N L−1). Overall, this study demonstrated for the first time that acclimation of methanogenic communities to extreme ammonia levels in continuous AD process is possible, by developing a specialised acclimation AD microbiome.",
author = "Hailin Tian and Ioannis Fotidis and Enrico Mancini and Laura Treu and Ahmed Mahdy and Mercedes Ballesteros and Cristina Gonz{\'a}lez-Fern{\'a}ndez and Irini Angelidaki",
year = "2018",
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language = "English",
volume = "247",
pages = "616--623",
journal = "Bioresource Technology",
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Acclimation to extremely high ammonia levels in continuous biomethanation process and the associated microbial community dynamics. / Tian, Hailin; Fotidis, Ioannis ; Mancini, Enrico; Treu, Laura; Mahdy, Ahmed; Ballesteros, Mercedes; González-Fernández, Cristina; Angelidaki, Irini.

In: Bioresource Technology, Vol. 247, 2018, p. 616-623.

Research output: Contribution to journalJournal articleResearchpeer-review

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T1 - Acclimation to extremely high ammonia levels in continuous biomethanation process and the associated microbial community dynamics

AU - Tian, Hailin

AU - Fotidis, Ioannis

AU - Mancini, Enrico

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AU - Mahdy, Ahmed

AU - Ballesteros, Mercedes

AU - González-Fernández, Cristina

AU - Angelidaki, Irini

PY - 2018

Y1 - 2018

N2 - Acclimatized anaerobic communities to high ammonia levels can offer a solution to the ammonia toxicity problem in biogas reactors. In the current study, a stepwise acclimation strategy up to 10 g NH4+-N L−1, was performed in mesophilic (37 ± 1 °C) continuously stirred tank reactors. The reactors were co-digesting (20/80 based on volatile solid) cattle slurry and microalgae, a protein-rich, 3rd generation biomass. Throughout the acclimation period, methane production was stable with more than 95% of the uninhibited yield. Next generation 16S rRNA gene sequencing revealed a dramatic microbiome change throughout the ammonia acclimation process. Clostridium ultunense, a syntrophic acetate oxidizing bacteria, increased significantly alongside with hydrogenotrophic methanogen Methanoculleus spp., indicating strong hydrogenotrophic methanogenic activity at extreme ammonia levels (>7 g NH4+-N L−1). Overall, this study demonstrated for the first time that acclimation of methanogenic communities to extreme ammonia levels in continuous AD process is possible, by developing a specialised acclimation AD microbiome.

AB - Acclimatized anaerobic communities to high ammonia levels can offer a solution to the ammonia toxicity problem in biogas reactors. In the current study, a stepwise acclimation strategy up to 10 g NH4+-N L−1, was performed in mesophilic (37 ± 1 °C) continuously stirred tank reactors. The reactors were co-digesting (20/80 based on volatile solid) cattle slurry and microalgae, a protein-rich, 3rd generation biomass. Throughout the acclimation period, methane production was stable with more than 95% of the uninhibited yield. Next generation 16S rRNA gene sequencing revealed a dramatic microbiome change throughout the ammonia acclimation process. Clostridium ultunense, a syntrophic acetate oxidizing bacteria, increased significantly alongside with hydrogenotrophic methanogen Methanoculleus spp., indicating strong hydrogenotrophic methanogenic activity at extreme ammonia levels (>7 g NH4+-N L−1). Overall, this study demonstrated for the first time that acclimation of methanogenic communities to extreme ammonia levels in continuous AD process is possible, by developing a specialised acclimation AD microbiome.

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