Abstract
| Original language | English |
|---|---|
| Journal | Bioresource Technology |
| Volume | 247 |
| Pages (from-to) | 616-623 |
| ISSN | 0960-8524 |
| DOIs | |
| Publication status | Published - 2018 |
Cite this
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Acclimation to extremely high ammonia levels in continuous biomethanation process and the associated microbial community dynamics. / Tian, Hailin; Fotidis, Ioannis ; Mancini, Enrico; Treu, Laura; Mahdy, Ahmed; Ballesteros, Mercedes; González-Fernández, Cristina; Angelidaki, Irini.
In: Bioresource Technology, Vol. 247, 2018, p. 616-623.Research output: Contribution to journal › Journal article › Research › peer-review
TY - JOUR
T1 - Acclimation to extremely high ammonia levels in continuous biomethanation process and the associated microbial community dynamics
AU - Tian, Hailin
AU - Fotidis, Ioannis
AU - Mancini, Enrico
AU - Treu, Laura
AU - Mahdy, Ahmed
AU - Ballesteros, Mercedes
AU - González-Fernández, Cristina
AU - Angelidaki, Irini
PY - 2018
Y1 - 2018
N2 - Acclimatized anaerobic communities to high ammonia levels can offer a solution to the ammonia toxicity problem in biogas reactors. In the current study, a stepwise acclimation strategy up to 10 g NH4+-N L−1, was performed in mesophilic (37 ± 1 °C) continuously stirred tank reactors. The reactors were co-digesting (20/80 based on volatile solid) cattle slurry and microalgae, a protein-rich, 3rd generation biomass. Throughout the acclimation period, methane production was stable with more than 95% of the uninhibited yield. Next generation 16S rRNA gene sequencing revealed a dramatic microbiome change throughout the ammonia acclimation process. Clostridium ultunense, a syntrophic acetate oxidizing bacteria, increased significantly alongside with hydrogenotrophic methanogen Methanoculleus spp., indicating strong hydrogenotrophic methanogenic activity at extreme ammonia levels (>7 g NH4+-N L−1). Overall, this study demonstrated for the first time that acclimation of methanogenic communities to extreme ammonia levels in continuous AD process is possible, by developing a specialised acclimation AD microbiome.
AB - Acclimatized anaerobic communities to high ammonia levels can offer a solution to the ammonia toxicity problem in biogas reactors. In the current study, a stepwise acclimation strategy up to 10 g NH4+-N L−1, was performed in mesophilic (37 ± 1 °C) continuously stirred tank reactors. The reactors were co-digesting (20/80 based on volatile solid) cattle slurry and microalgae, a protein-rich, 3rd generation biomass. Throughout the acclimation period, methane production was stable with more than 95% of the uninhibited yield. Next generation 16S rRNA gene sequencing revealed a dramatic microbiome change throughout the ammonia acclimation process. Clostridium ultunense, a syntrophic acetate oxidizing bacteria, increased significantly alongside with hydrogenotrophic methanogen Methanoculleus spp., indicating strong hydrogenotrophic methanogenic activity at extreme ammonia levels (>7 g NH4+-N L−1). Overall, this study demonstrated for the first time that acclimation of methanogenic communities to extreme ammonia levels in continuous AD process is possible, by developing a specialised acclimation AD microbiome.
U2 - 10.1016/j.biortech.2017.09.148
DO - 10.1016/j.biortech.2017.09.148
M3 - Journal article
C2 - 28985610
VL - 247
SP - 616
EP - 623
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
ER -