Accelerated H-2 Evolution during Microbial Electrosynthesis with Sporomusa ovata

Pier-Luc Tremblay, Neda Faraghiparapari, Tian Zhang*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Microbial electrosynthesis (MES) is a process where bacteria acquire electrons from a cathode to convert CO2 into multicarbon compounds or methane. In MES with Sporomusa ovata as the microbial catalyst, cathode potential has often been used as a benchmark to determine whether electron uptake is hydrogen-dependent. In this study, H-2 was detected by a microsensor in proximity to the cathode. With a sterile fresh medium, H-2 was produced at a potential of -700 mV versus Ag/AgCl, whereas H-2 was detected at -500 mV versus Ag/AgCl with cell-free spent medium from a S. ovata culture. Furthermore, H-2 evolution rates were increased with potentials lower than -500 mV in the presence of cell-free spent medium in the cathode chamber. Nickel and cobalt were detected at the cathode surface after exposure to the spent medium, suggesting a possible participation of these catalytic metals in the observed faster hydrogen evolution. The results presented here show that S. ovata-induced alterations of the cathodic electrolytes of a MES reactor reduced the electrical energy required for hydrogen evolution. These observations also indicated that, even at higher cathode potentials, at least a part of the electrons coming from the electrode are transferred to S. ovata via H-2 during MES.
Original languageEnglish
Article number166
JournalCatalysts
Volume9
Issue number2
ISSN2073-4344
DOIs
Publication statusPublished - 2019

Keywords

  • industrial biotechnology
  • electrochemistry
  • biohydrogen
  • biocatalysis
  • process development
  • bacteria

Cite this

Tremblay, Pier-Luc ; Faraghiparapari, Neda ; Zhang, Tian. / Accelerated H-2 Evolution during Microbial Electrosynthesis with Sporomusa ovata. In: Catalysts. 2019 ; Vol. 9, No. 2.
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abstract = "Microbial electrosynthesis (MES) is a process where bacteria acquire electrons from a cathode to convert CO2 into multicarbon compounds or methane. In MES with Sporomusa ovata as the microbial catalyst, cathode potential has often been used as a benchmark to determine whether electron uptake is hydrogen-dependent. In this study, H-2 was detected by a microsensor in proximity to the cathode. With a sterile fresh medium, H-2 was produced at a potential of -700 mV versus Ag/AgCl, whereas H-2 was detected at -500 mV versus Ag/AgCl with cell-free spent medium from a S. ovata culture. Furthermore, H-2 evolution rates were increased with potentials lower than -500 mV in the presence of cell-free spent medium in the cathode chamber. Nickel and cobalt were detected at the cathode surface after exposure to the spent medium, suggesting a possible participation of these catalytic metals in the observed faster hydrogen evolution. The results presented here show that S. ovata-induced alterations of the cathodic electrolytes of a MES reactor reduced the electrical energy required for hydrogen evolution. These observations also indicated that, even at higher cathode potentials, at least a part of the electrons coming from the electrode are transferred to S. ovata via H-2 during MES.",
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Accelerated H-2 Evolution during Microbial Electrosynthesis with Sporomusa ovata. / Tremblay, Pier-Luc; Faraghiparapari, Neda; Zhang, Tian.

In: Catalysts, Vol. 9, No. 2, 166, 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Accelerated H-2 Evolution during Microbial Electrosynthesis with Sporomusa ovata

AU - Tremblay, Pier-Luc

AU - Faraghiparapari, Neda

AU - Zhang, Tian

PY - 2019

Y1 - 2019

N2 - Microbial electrosynthesis (MES) is a process where bacteria acquire electrons from a cathode to convert CO2 into multicarbon compounds or methane. In MES with Sporomusa ovata as the microbial catalyst, cathode potential has often been used as a benchmark to determine whether electron uptake is hydrogen-dependent. In this study, H-2 was detected by a microsensor in proximity to the cathode. With a sterile fresh medium, H-2 was produced at a potential of -700 mV versus Ag/AgCl, whereas H-2 was detected at -500 mV versus Ag/AgCl with cell-free spent medium from a S. ovata culture. Furthermore, H-2 evolution rates were increased with potentials lower than -500 mV in the presence of cell-free spent medium in the cathode chamber. Nickel and cobalt were detected at the cathode surface after exposure to the spent medium, suggesting a possible participation of these catalytic metals in the observed faster hydrogen evolution. The results presented here show that S. ovata-induced alterations of the cathodic electrolytes of a MES reactor reduced the electrical energy required for hydrogen evolution. These observations also indicated that, even at higher cathode potentials, at least a part of the electrons coming from the electrode are transferred to S. ovata via H-2 during MES.

AB - Microbial electrosynthesis (MES) is a process where bacteria acquire electrons from a cathode to convert CO2 into multicarbon compounds or methane. In MES with Sporomusa ovata as the microbial catalyst, cathode potential has often been used as a benchmark to determine whether electron uptake is hydrogen-dependent. In this study, H-2 was detected by a microsensor in proximity to the cathode. With a sterile fresh medium, H-2 was produced at a potential of -700 mV versus Ag/AgCl, whereas H-2 was detected at -500 mV versus Ag/AgCl with cell-free spent medium from a S. ovata culture. Furthermore, H-2 evolution rates were increased with potentials lower than -500 mV in the presence of cell-free spent medium in the cathode chamber. Nickel and cobalt were detected at the cathode surface after exposure to the spent medium, suggesting a possible participation of these catalytic metals in the observed faster hydrogen evolution. The results presented here show that S. ovata-induced alterations of the cathodic electrolytes of a MES reactor reduced the electrical energy required for hydrogen evolution. These observations also indicated that, even at higher cathode potentials, at least a part of the electrons coming from the electrode are transferred to S. ovata via H-2 during MES.

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KW - process development

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