Abundance of specific mRNA transcripts impacts hatching success in European eel, Anguilla anguilla L

Christoffer Rozenfeld, Ian A.E. Butts, Jonna Tomkiewicz, Jose-Luis Zambonino-Infante, David Mazurais

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

MaternalmRNA governs earlyembryonic development in fish and variation in abundance of maternal transcripts may contribute to variation in embryonic survival and hatch success in European eel, Anguilla anguilla. Previous
studies have shown that quantities of the maternal gene products β-tubulin, insulin-like growth factor 2 (igf2), nucleoplasmin (npm2), prohibitin 2 (phb2), phosphatidylinositol glycan biosynthesis class F protein 5 (pigf5), and carnitine O-palmitoyltransferase liver isoform-like 1 (cpt1) are associated with embryonic developmental competence in other teleosts. Here, the relations between relative mRNA abundance of these genes in eggs and/or embryos and egg quality, was studied and analyzed. We compared egg quality of the two groups: i) batches with hatching and ii) batches with no hatching. Results showed no significant differences in relative mRNA abundance between the hatch and no hatching groups for any of the selected genes at 0, 2.5, and 5 HPF.
However, at 30 HPF the hatch group showed significantly higher abundance of cpt1a, cpt1b, β-tubulin, phb2, and pigf5 transcripts than the no hatch group. Therefore, these results indicate that up-regulation of the transcription
of these genes in European eel after the mid-blastula transition, may be needed to sustain embryonic development and hatching success
Original languageEnglish
JournalComparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology
Volume191
Pages (from-to)59-65
ISSN1095-6433
DOIs
Publication statusPublished - 2016

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