Abundance of specific mRNA transcripts impacts hatching success in European eel, Anguilla anguilla L

Christoffer Rozenfeld, Ian A.E. Butts, Jonna Tomkiewicz, Jose-Luis Zambonino-Infante, David Mazurais

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

MaternalmRNA governs earlyembryonic development in fish and variation in abundance of maternal transcripts may contribute to variation in embryonic survival and hatch success in European eel, Anguilla anguilla. Previous
studies have shown that quantities of the maternal gene products β-tubulin, insulin-like growth factor 2 (igf2), nucleoplasmin (npm2), prohibitin 2 (phb2), phosphatidylinositol glycan biosynthesis class F protein 5 (pigf5), and carnitine O-palmitoyltransferase liver isoform-like 1 (cpt1) are associated with embryonic developmental competence in other teleosts. Here, the relations between relative mRNA abundance of these genes in eggs and/or embryos and egg quality, was studied and analyzed. We compared egg quality of the two groups: i) batches with hatching and ii) batches with no hatching. Results showed no significant differences in relative mRNA abundance between the hatch and no hatching groups for any of the selected genes at 0, 2.5, and 5 HPF.
However, at 30 HPF the hatch group showed significantly higher abundance of cpt1a, cpt1b, β-tubulin, phb2, and pigf5 transcripts than the no hatch group. Therefore, these results indicate that up-regulation of the transcription
of these genes in European eel after the mid-blastula transition, may be needed to sustain embryonic development and hatching success
Original languageEnglish
JournalComparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology
Volume191
Pages (from-to)59-65
ISSN1095-6433
DOIs
Publication statusPublished - 2016

Cite this

@article{27ba7a0c73b04a9e845ca22566a29f9f,
title = "Abundance of specific mRNA transcripts impacts hatching success in European eel, Anguilla anguilla L",
abstract = "MaternalmRNA governs earlyembryonic development in fish and variation in abundance of maternal transcripts may contribute to variation in embryonic survival and hatch success in European eel, Anguilla anguilla. Previousstudies have shown that quantities of the maternal gene products β-tubulin, insulin-like growth factor 2 (igf2), nucleoplasmin (npm2), prohibitin 2 (phb2), phosphatidylinositol glycan biosynthesis class F protein 5 (pigf5), and carnitine O-palmitoyltransferase liver isoform-like 1 (cpt1) are associated with embryonic developmental competence in other teleosts. Here, the relations between relative mRNA abundance of these genes in eggs and/or embryos and egg quality, was studied and analyzed. We compared egg quality of the two groups: i) batches with hatching and ii) batches with no hatching. Results showed no significant differences in relative mRNA abundance between the hatch and no hatching groups for any of the selected genes at 0, 2.5, and 5 HPF.However, at 30 HPF the hatch group showed significantly higher abundance of cpt1a, cpt1b, β-tubulin, phb2, and pigf5 transcripts than the no hatch group. Therefore, these results indicate that up-regulation of the transcriptionof these genes in European eel after the mid-blastula transition, may be needed to sustain embryonic development and hatching success",
author = "Christoffer Rozenfeld and Butts, {Ian A.E.} and Jonna Tomkiewicz and Jose-Luis Zambonino-Infante and David Mazurais",
year = "2016",
doi = "10.1016/j.cbpa.2015.09.011",
language = "English",
volume = "191",
pages = "59--65",
journal = "Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology",
issn = "1095-6433",
publisher = "Elsevier",

}

Abundance of specific mRNA transcripts impacts hatching success in European eel, Anguilla anguilla L. / Rozenfeld, Christoffer; Butts, Ian A.E.; Tomkiewicz, Jonna; Zambonino-Infante, Jose-Luis; Mazurais, David.

In: Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, Vol. 191, 2016, p. 59-65.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Abundance of specific mRNA transcripts impacts hatching success in European eel, Anguilla anguilla L

AU - Rozenfeld, Christoffer

AU - Butts, Ian A.E.

AU - Tomkiewicz, Jonna

AU - Zambonino-Infante, Jose-Luis

AU - Mazurais, David

PY - 2016

Y1 - 2016

N2 - MaternalmRNA governs earlyembryonic development in fish and variation in abundance of maternal transcripts may contribute to variation in embryonic survival and hatch success in European eel, Anguilla anguilla. Previousstudies have shown that quantities of the maternal gene products β-tubulin, insulin-like growth factor 2 (igf2), nucleoplasmin (npm2), prohibitin 2 (phb2), phosphatidylinositol glycan biosynthesis class F protein 5 (pigf5), and carnitine O-palmitoyltransferase liver isoform-like 1 (cpt1) are associated with embryonic developmental competence in other teleosts. Here, the relations between relative mRNA abundance of these genes in eggs and/or embryos and egg quality, was studied and analyzed. We compared egg quality of the two groups: i) batches with hatching and ii) batches with no hatching. Results showed no significant differences in relative mRNA abundance between the hatch and no hatching groups for any of the selected genes at 0, 2.5, and 5 HPF.However, at 30 HPF the hatch group showed significantly higher abundance of cpt1a, cpt1b, β-tubulin, phb2, and pigf5 transcripts than the no hatch group. Therefore, these results indicate that up-regulation of the transcriptionof these genes in European eel after the mid-blastula transition, may be needed to sustain embryonic development and hatching success

AB - MaternalmRNA governs earlyembryonic development in fish and variation in abundance of maternal transcripts may contribute to variation in embryonic survival and hatch success in European eel, Anguilla anguilla. Previousstudies have shown that quantities of the maternal gene products β-tubulin, insulin-like growth factor 2 (igf2), nucleoplasmin (npm2), prohibitin 2 (phb2), phosphatidylinositol glycan biosynthesis class F protein 5 (pigf5), and carnitine O-palmitoyltransferase liver isoform-like 1 (cpt1) are associated with embryonic developmental competence in other teleosts. Here, the relations between relative mRNA abundance of these genes in eggs and/or embryos and egg quality, was studied and analyzed. We compared egg quality of the two groups: i) batches with hatching and ii) batches with no hatching. Results showed no significant differences in relative mRNA abundance between the hatch and no hatching groups for any of the selected genes at 0, 2.5, and 5 HPF.However, at 30 HPF the hatch group showed significantly higher abundance of cpt1a, cpt1b, β-tubulin, phb2, and pigf5 transcripts than the no hatch group. Therefore, these results indicate that up-regulation of the transcriptionof these genes in European eel after the mid-blastula transition, may be needed to sustain embryonic development and hatching success

U2 - 10.1016/j.cbpa.2015.09.011

DO - 10.1016/j.cbpa.2015.09.011

M3 - Journal article

VL - 191

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EP - 65

JO - Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology

JF - Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology

SN - 1095-6433

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