Absolute yeast mitochondrial proteome quantification reveals trade-off between biosynthesis and energy generation during diauxic shift

Francesca Di Bartolomeo, Carl Malina, Kate Campbell, Maurizio Mormino, Johannes Fuchs, Egor Vorontsov, Claes M. Gustafsson, Jens Nielsen

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Abstract

Saccharomyces cerevisiae constitutes a popular eukaryal model for research on mitochondrial physiology. Being Crabtree-positive, this yeast has evolved the ability to ferment glucose to ethanol and respire ethanol once glucose is consumed. Its transition phase from fermentative to respiratory metabolism, known as the diauxic shift, is reflected by dramatic rearrangements of mitochondrial function and structure. To date, the metabolic adaptations that occur during the diauxic shift have not been fully characterized at the organelle level. In this study, the absolute proteome of mitochondria was quantified alongside precise parametrization of biophysical properties associated with the mitochondrial network using state-of-the-art optical-imaging techniques. This allowed the determination of absolute protein abundances at a subcellular level. By tracking the transformation of mitochondrial mass and volume, alongside changes in the absolute mitochondrial proteome allocation, we could quantify how mitochondria balance their dual role as a biosynthetic hub as well as a center for cellular respiration. Furthermore, our findings suggest that in the transition from a fermentative to a respiratory metabolism, the diauxic shift represents the stage where major structural and functional reorganizations in mitochondrial metabolism occur. This metabolic transition, initiated at the mitochondria level, is then extended to the rest of the yeast cell.
Original languageEnglish
JournalProceedings of the National Academy of Sciences of the United States of America
Volume117
Issue number13
Pages (from-to)7524-7535
ISSN0027-8424
DOIs
Publication statusPublished - 2020

Keywords

  • Mitochondria
  • Absolute proteomics
  • Saccharomyces cerevisiae
  • Diauxic shift

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