A versatile expression vector system for mammalian cell factories

Anne Mathilde Lund; Helene Faustrup Kildegaard; Bjarne Gram Hansen; Mikael Rørdam Andersen; Uffe Hasbro Mortensen

A versatile expression vector system for mammalian cell factories

Anne Mathilde Lund, Helene Faustrup Kildegaard, Bjarne Gram Hansen, Mikael Rørdam Andersen, Uffe Hasbro Mortensen

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Abstract

The development of the field of mammalian cell factories requests fast and high-throughput methods which means high need for simpler and more efficient cloning techniques. This project applies the ligation-free USERTM (uracil-specific excision reagent) cloning technique to construct mammalian expression vectors with maximum flexibility.

Original language	English
Publication date	2011
Publication status	Published - 2011
Event	6th Danish Conference on Biotechnology and Molecular Biology: Synthetic Biology and Cell Factories - Vejle, Denmark Duration: 26 May 2011 → 27 May 2011 Conference number: 6

Conference

Conference	6th Danish Conference on Biotechnology and Molecular Biology
Number	6
Country/Territory	Denmark
City	Vejle
Period	26/05/2011 → 27/05/2011

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title = "A versatile expression vector system for mammalian cell factories",

abstract = "The development of the field of mammalian cell factories requests fast and high-throughput methods which means high need for simpler and more efficient cloning techniques. This project applies the ligation-free USERTM (uracil-specific excision reagent) cloning technique to construct mammalian expression vectors with maximum flexibility.",

author = "Lund, {Anne Mathilde} and Kildegaard, {Helene Faustrup} and Hansen, {Bjarne Gram} and Andersen, {Mikael R{\o}rdam} and Mortensen, {Uffe Hasbro}",

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AU - Lund, Anne Mathilde

AU - Kildegaard, Helene Faustrup

AU - Hansen, Bjarne Gram

AU - Andersen, Mikael Rørdam

AU - Mortensen, Uffe Hasbro

N1 - Conference code: 6

PY - 2011

Y1 - 2011

N2 - The development of the field of mammalian cell factories requests fast and high-throughput methods which means high need for simpler and more efficient cloning techniques. This project applies the ligation-free USERTM (uracil-specific excision reagent) cloning technique to construct mammalian expression vectors with maximum flexibility.

AB - The development of the field of mammalian cell factories requests fast and high-throughput methods which means high need for simpler and more efficient cloning techniques. This project applies the ligation-free USERTM (uracil-specific excision reagent) cloning technique to construct mammalian expression vectors with maximum flexibility.

M3 - Poster

T2 - 6th Danish Conference on Biotechnology and Molecular Biology

Y2 - 26 May 2011 through 27 May 2011

ER -

A versatile expression vector system for mammalian cell factories

Abstract

Conference

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