A versatile expression vector system for mammalian cell factories

Anne Mathilde Lund, Helene Faustrup Kildegaard, Bjarne Gram Hansen, Mikael Rørdam Andersen, Uffe Hasbro Mortensen

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    Abstract

    The development of the field of mammalian cell factories requests fast and high-throughput methods which means high need for simpler and more efficient cloning techniques. This project applies the ligation-free USERTM (uracil-specific excision reagent) cloning technique to construct mammalian expression vectors with maximum flexibility.
    Original languageEnglish
    Publication date2011
    Publication statusPublished - 2011
    Event6th Danish Conference on Biotechnology and Molecular Biology: Synthetic Biology and Cell Factories - Vejle, Denmark
    Duration: 26 May 201127 May 2011
    Conference number: 6

    Conference

    Conference6th Danish Conference on Biotechnology and Molecular Biology
    Number6
    CountryDenmark
    CityVejle
    Period26/05/201127/05/2011

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